Haga S, Aizawa T, Ishii T, Ikeda K
Department of Ultrastructure and Histochemistry, Tokyo Institute of Psychiatry, Japan.
Neurosci Lett. 1996 Oct 4;216(3):191-4. doi: 10.1016/0304-3940(96)13040-8.
We investigated the mRNA expression of the various complement components in cultured mouse microglia and astrocytes by the reverse transcription and polymerase chain reaction. C1q, C2, C3, and C4 mRNAs were detected in microglial cultures. C3 and C4 mRNAs were found in astrocyte cultures. Microglia showed enhanced expression of C2, C3, and C4 mRNAs when they were treated with lipopolysaccharide. Much higher expression of C1q, C2, C3, and C4 mRNAs was detected in microglia after stimulation with interferon-gamma. Our data suggest that microglia and astrocytes may produce some of the complement components also in vivo, which can be facilitated in certain infectious and inflammatory diseases in the central nervous system.
我们通过逆转录和聚合酶链反应研究了培养的小鼠小胶质细胞和星形胶质细胞中各种补体成分的mRNA表达。在小胶质细胞培养物中检测到C1q、C2、C3和C4的mRNA。在星形胶质细胞培养物中发现了C3和C4的mRNA。当用脂多糖处理时，小胶质细胞中C2、C3和C4的mRNA表达增强。在用γ干扰素刺激后，小胶质细胞中检测到C1q、C2、C3和C4的mRNA表达水平更高。我们的数据表明，小胶质细胞和星形胶质细胞在体内也可能产生一些补体成分，在中枢神经系统的某些感染性和炎症性疾病中可能会促进这种产生。
