Department of Ophthalmology, Emory University , Atlanta, Georgia .
J Neurotrauma. 2018 Jan 1;35(1):118-129. doi: 10.1089/neu.2017.5104. Epub 2017 Aug 18.
Ocular blast injury is a major medical concern for soldiers and explosion victims due to poor visual outcomes. To define the changes in gene expression following a blast injury to the eye, we examined retinal ribonucleic acid (RNA) expression in 54 mouse strains 5 days after a single 50-psi overpressure air wave blast injury. We observe that almost 40% of genes are differentially expressed with a false discovery rate (FDR) of <0.001, even though the nominal changes in RNA expression are rather small. Moreover, we find through machine learning approaches that genetic networks related to the innate and acquired immune system are activated. Accompanied by lymphocyte invasion into the inner retina, blast injury also results in progressive loss of visual function and retinal ganglion cells (RGCs). Collectively, these data demonstrate how systems genetics can be used to put meaning to the transcriptome changes following ocular blast injury that eventually lead to blindness.
眼冲击伤是士兵和爆炸受害者的一个主要医学关注点，因为其视觉预后较差。为了明确眼部冲击伤后的基因表达变化，我们在单次 50psi 超压空气波冲击伤后 5 天检查了 54 种小鼠品系的视网膜核糖核酸（RNA）表达。我们观察到，即使 RNA 表达的名义变化很小，近 40%的基因的表达具有统计学差异（假发现率<0.001）。此外，我们通过机器学习方法发现，与先天和获得性免疫系统相关的基因网络被激活。伴随淋巴细胞侵入内视网膜，冲击伤也导致视觉功能和视网膜神经节细胞（RGC）的进行性丧失。总的来说，这些数据表明系统遗传学如何能够为眼部冲击伤后导致失明的转录组变化赋予意义。
