Declich P, Isimbaldi G, Sironi M, Galli C, Ferrara A, Caruso S, Baldacci M P, Stioui S, Privitera O, Boccazzi G, Federici S
Department of Pathology, Legnano General Hospital, Italy.
Pathol Res Pract. 1996 Aug;192(8):808-15. doi: 10.1016/S0344-0338(96)80054-9.
Fundic Gland Polyps (FGPs) are small sessile (2-5 mm), usually multiple polyps arising in the gastric, acid-secreting mucosa of disputed histogenesis. They have been described in a sporadic form, prevalently in middle aged females, or associated with familial adenomatosis coli-Gardner's syndrome. We performed an immunohistochemical study on 24 sporadic FGPs, using monoclonal antibodies (MAbs) against differentiation markers, class II MHC antigens (HLA-DR), oncofetal and proliferation antigens, aimed to characterize the antigenic profile of the polyps. A preliminary cytogenetic study on five polyps was also done, using an in situ culture method after collagenase treatment. Cytokeratins 8-18 (CAM 5.2 MAb) and 20 (IT-Ks 20.8 MAb), Epithelial Membrane Antigen (EMA) and Chromogranin A were normally expressed by FGPs. FGPs did not express HLA II DR. FGPs did not react with an anti-CEA MAb (F6), but they were frequently positive (22/24, 91.6%) with B72.3 MAb (reacting with the cancer-associated mucin epitope sialyl-Tn). The PC10 MAb (against PCNA or cyclin) showed enhanced expression in the deep glandular-cystic compartment of FGPs; the PCNA index of FGPs was significantly higher than in normal fundic mucosa. The cytogenetic study on the 5 cases analysed, revealed a normal karyotype. We have demonstrated that FGPs express in the paranuclear zone the sialyl-Tn epitope, a side-chain sugar normally masqued in adult gastric mucins, thus revealing an alteration in mucin synthesis; FGPs' higher proliferation index as compared with normal fundic mucosa supports the hypothesis of their hyperproliferative nature.
胃底腺息肉（FGPs）是小的无柄息肉（2 - 5毫米），通常为多个，发生于胃的泌酸黏膜，其组织发生存在争议。它们以散发性形式被描述，多见于中年女性，或与家族性腺瘤性结肠息肉病 - 加德纳综合征相关。我们使用针对分化标志物、II类MHC抗原（HLA - DR）、癌胚和增殖抗原的单克隆抗体（MAbs），对24例散发性胃底腺息肉进行了免疫组织化学研究，旨在描述息肉的抗原谱。还对5个息肉进行了初步细胞遗传学研究，采用胶原酶处理后的原位培养方法。细胞角蛋白8 - 18（CAM 5.2 MAb）和20（IT - Ks 20.8 MAb）、上皮膜抗原（EMA）和嗜铬粒蛋白A在胃底腺息肉中正常表达。胃底腺息肉不表达HLA II DR。胃底腺息肉与抗CEA MAb（F6）不反应，但它们经常与B72.3 MAb呈阳性反应（22/24，91.6%）（与癌相关黏蛋白表位唾液酸 - Tn反应）。PC10 MAb（针对PCNA或细胞周期蛋白）在胃底腺息肉的深部腺囊性区域表达增强；胃底腺息肉的PCNA指数显著高于正常胃底黏膜。对分析的5例病例的细胞遗传学研究显示核型正常。我们已经证明，胃底腺息肉在核旁区表达唾液酸 - Tn表位，这是一种在成人胃黏蛋白中通常被掩盖的侧链糖，从而揭示了黏蛋白合成的改变；与正常胃底黏膜相比，胃底腺息肉较高的增殖指数支持了其增殖性过高的假说。
