Soszyński M, Bartosz G
Department of Molecular Biophysics, University of Lodź, Poland.
Free Radic Biol Med. 1997;23(3):463-9. doi: 10.1016/s0891-5849(97)00117-2.
The effect of several oxidative agents (hydrogen peroxide, tert-butyl hydroperoxide, menadione, AAPH, peroxynitrite and ionizing radiation) on the ratio of weakly to strongly immobilized residues of erythrocyte membrane-bound maleimide-tempo spin label (h(w)/h(s) ratio) was studied in order to test the hypothesis that a decrease in the h(w)/h(s) ratio may be a general index of oxidative damage to membrane proteins. Most of the agents studied decreased though H2O2 almost did not affect and ionizing radiation increased the h(w)/h(s) ratio. In parallel, the ratio of DTNB accessible/DTNB inaccessible membrane protein-SH groups was determined from membrane-SH group measurements with the Ellman reagent in the absence and in the presence of sodium dodecyl sulfate. This ratio decreased in all cases studied and seems to be a more universal and easy to measure parameter to describe the oxidative damage to membrane proteins.
为了验证红细胞膜结合的马来酰亚胺 - 四甲基哌啶氧化物自旋标记物中弱固定与强固定残基的比例（h(w)/h(s) 比值）的降低可能是膜蛋白氧化损伤的一个通用指标这一假设，研究了几种氧化剂（过氧化氢、叔丁基过氧化氢、甲萘醌、2,2'-偶氮二异丁腈、过氧亚硝酸盐和电离辐射）对该比值的影响。所研究的大多数试剂会使h(w)/h(s) 比值降低，不过过氧化氢几乎没有影响，而电离辐射会使h(w)/h(s) 比值升高。同时，在不存在和存在十二烷基硫酸钠的情况下，用埃尔曼试剂通过测量膜 - SH基团来确定二硫代硝基苯甲酸（DTNB）可及/不可及的膜蛋白 - SH基团的比例。在所研究的所有情况下，该比例均降低，并且似乎是一个更通用且易于测量的参数，可用于描述膜蛋白的氧化损伤。
