Chonan O, Watanuki M
Yakult Central Institute for Microbiological Research, Tokyo, Japan.
Int J Vitam Nutr Res. 1996;66(3):244-9.
6'-galactooligosaccharides (6'-GOS), a mixture of galactosyl oligosaccharides formed from lactose by the transgalactosyl reaction with beta-D-galactosidase derived from Aspergillus oryzae and Streptococcus thermophillus, are unhydrolyzed in the small intestine and are fermented by the intestinal bacteria. The effects of 6'-GOS on calcium (Ca) absorption and bone mineralization were examined in male Wistar rats adapted to different levels of dietary Ca for 30 days. Dietary 6'-GOS (5 g/100 g of diet) were more potent than control in stimulating Ca absorption in rats fed the Normal-Ca diet (0.5 g of Ca/100 g of diet) after 8-10 days and 18-20 days, and the bone (femur and tibia) Ca content of rats fed the Normal-Ca diet with 6'-GOS were significantly higher than those of the control animals. However, in rats fed the Low-Ca diet (0.05 g of Ca/100 g of diet), 6'-GOS feeding did not affect both the absorption of Ca and the bone mineralization. Ca content in the liquid phase of the cecal digesta was significantly elevated by 6'-GOS feeding in the rats fed the Normal-Ca diet, however, this was unchanged in the rats fed the Low-Ca diet. We conclude that the effect of 6'-GOS on the bone mineralization is affected by dietary Ca concentration used in the experiment, and the stimulatory effect of 6'-GOS on Ca absorption may be partly associated with increased solubility of Ca in the intestinal digesta.
6'-低聚半乳糖（6'-GOS）是乳糖通过与源自米曲霉和嗜热链球菌的β-D-半乳糖苷酶进行转半乳糖基反应形成的半乳糖基低聚糖混合物，在小肠中不被水解，而是由肠道细菌发酵。在适应不同膳食钙水平30天的雄性Wistar大鼠中，研究了6'-GOS对钙（Ca）吸收和骨矿化的影响。在8 - 10天和18 - 20天后，在喂食正常钙饮食（0.5 g钙/100 g饮食）的大鼠中，膳食6'-GOS（5 g/100 g饮食）在刺激钙吸收方面比对照组更有效，并且喂食含6'-GOS的正常钙饮食的大鼠的骨骼（股骨和胫骨）钙含量显著高于对照动物。然而，在喂食低钙饮食（0.05 g钙/100 g饮食）的大鼠中，喂食6'-GOS对钙吸收和骨矿化均无影响。在喂食正常钙饮食的大鼠中，6'-GOS喂食显著提高了盲肠消化物液相中的钙含量，然而，在喂食低钙饮食的大鼠中，这一情况未发生变化。我们得出结论，6'-GOS对骨矿化的影响受实验中使用的膳食钙浓度影响，并且6'-GOS对钙吸收的刺激作用可能部分与肠道消化物中钙溶解度的增加有关。
