Kovacheva S, Ribarov S
Department of Biophysics, Sofia University of Medicine, Bulgaria.
Pharmacol Toxicol. 1996 Oct;79(4):177-82. doi: 10.1111/j.1600-0773.1996.tb02085.x.
The aim of this work was to investigate the possibility for development of peroxidation in the lung of rats injected with endotoxin. The effect of vitamin E supplementation of the rats was also investigated. Vitamin E supplementation by itself did not cause visible differences or any pathological changes in the lung structure of rats as shown by routine histological examinations. The endotoxin-induced alterations of the lung structures were well expressed in vitamin E-non-supplemented rats, while they were rather negligible in vitamin E-supplemented rats. It was established also that endotoxin caused a 50% increase of the conjugated dienes in the lung of vitamin E-non-supplemented rats. For vitamin E-supplemented rats this increase was less than 13.5%. The fluorescent lipofuscine-like products of lipid peroxidation (known as one of the end products of lipid peroxidation) increased by 120% in vitamin E-non-supplemented rats after exposure to endotoxin, while in vitamin E-supplemented rats this increase did not exceed 26%. Exposure to endotoxin of both vitamin E-non-supplemented and vitamin E-supplemented rats did not lead to significant changes of the lung fatty acid composition. On the basis of these results we assumed that the endotoxin-induced changes of lung structures involve, at least in part, free radical-mediated damage of the lung membrane lipids and proteins.
这项工作的目的是研究注射内毒素的大鼠肺部发生过氧化的可能性。同时也研究了给大鼠补充维生素E的效果。常规组织学检查显示，单独补充维生素E不会在大鼠肺部结构上引起明显差异或任何病理变化。内毒素引起的肺部结构改变在未补充维生素E的大鼠中表现明显，而在补充维生素E的大鼠中则相当不明显。还发现，内毒素使未补充维生素E的大鼠肺部共轭二烯增加了50%。对于补充维生素E的大鼠，这种增加小于13.5%。脂质过氧化的荧光脂褐素样产物（已知为脂质过氧化的终产物之一）在未补充维生素E的大鼠接触内毒素后增加了120%，而在补充维生素E的大鼠中，这种增加不超过26%。未补充维生素E和补充维生素E的大鼠接触内毒素均未导致肺脂肪酸组成的显著变化。基于这些结果，我们推测内毒素引起的肺部结构变化至少部分涉及自由基介导的肺膜脂质和蛋白质损伤。
