Ragan M A, Logsdon J M, Sensen C W, Charlebois R L, Doolittle W F
Program in Evolutionary Biology, Canadian Institue for Advanced Research, National Research Council of Canada, Halifax, Nova Scotia, Canada.
FEMS Microbiol Lett. 1996 Nov 1;144(2-3):151-5. doi: 10.1111/j.1574-6968.1996.tb08522.x.
An open reading frame (pelA) specifying a homolog of pelota and DOM34, proteins required for meiotic cell division in Drosophila melanogaster and Saccharomyces cerevisiae, respectively, has been cloned, sequenced and identified from the archaebacterium Sulfolobus solfataricus. The S. solfataricus PelA protein is about 20% identical with pelota, DOM34 and the hypothetical protein R74.6 of Caenorhabditis elegans. The presence of a pelota homolog in archaebacteria implies that the meiotic functions of the eukaryotic protein were co-opted from, or added to, other functions existing before the emergence of eukaryotes. The nuclear localization signal and negatively charged carboxy-terminus characteristic of eukaryotic pelota-like proteins are absent from the S. solfataricus homolog, and hence may be indicative of the acquired eukaryotic function(s).
从嗜热栖热放线菌中克隆、测序并鉴定出一个开放阅读框（pelA），它编码一种与pelota和DOM34同源的蛋白，这两种蛋白分别是黑腹果蝇和酿酒酵母减数分裂细胞分裂所必需的。嗜热栖热放线菌的PelA蛋白与pelota、DOM34以及秀丽隐杆线虫的假想蛋白R74.6约有20%的同源性。古细菌中存在pelota同源物意味着真核生物蛋白的减数分裂功能是从真核生物出现之前就已存在的其他功能中衍生而来，或者是在这些功能基础上添加的。嗜热栖热放线菌的同源物中不存在真核生物pelota样蛋白所特有的核定位信号和带负电荷的羧基末端，因此这可能表明这些是真核生物后天获得的功能。
