Davis L, Engebrecht J
Department of Pharmacological Sciences and Graduate Program in Genetics, State University of New York, Stony Brook, New York 11794, USA.
Genetics. 1998 May;149(1):45-56. doi: 10.1093/genetics/149.1.45.
The DOM34 gene of Saccharomyces cerevisiae is similar to genes found in diverse eukaryotes and archaebacteria. Analysis of dom34 strains shows that progression through the G1 phase of the cell cycle is delayed, mutant cells enter meiosis aberrantly, and their ability to form pseudohyphae is significantly diminisehd. RPS30A, which encodes ribosomal protein S30, was identified in a screen for high-copy suppressors of the dom34delta growth defect. dom34delta mutants display an altered polyribosome profile that is rescued by expression of RPS30A. Taken together, these data indicate that Dom34p functions in protein translation to promote G1 progression and differentiation. A Drosophila homolog of Dom34p, pelota, is required for the proper coordination of meiosis and spermatogenesis. Heterologous expression of pelota in dom34delata mutants restores wild-type growth and differentiation, suggesting conservation of function between the eukaryotic members of the gene family.
酿酒酵母的DOM34基因与在多种真核生物和古细菌中发现的基因相似。对dom34菌株的分析表明，细胞周期G1期的进程延迟，突变细胞异常进入减数分裂，并且它们形成假菌丝的能力显著降低。在对dom34δ生长缺陷的高拷贝抑制子的筛选中，鉴定出了编码核糖体蛋白S30的RPS30A。dom34δ突变体表现出改变的多核糖体谱，而RPS30A的表达可使其恢复。综上所述，这些数据表明Dom34p在蛋白质翻译中发挥作用，以促进G1期进程和分化。Dom34p的果蝇同源物pelota是减数分裂和精子发生正常协调所必需的。pelota在dom34δ突变体中的异源表达恢复了野生型生长和分化，表明该基因家族的真核成员之间功能保守。
