Hulhoven R, Desager J P
Biomedicine. 1977 Apr;27(3):102-4.
A high-performance liquid chromatographic method for the quantitative simultaneous measurement of daunorubicin and its active metabolite, daunorubicinol, at low concentrations in human plasma was described. The advantages of this method were specificity, sensitivity, minimal pre-analysis work-up and small sample size. The method was sensitive for plasma levels of daunorubicin and daunorubicinol as low as 10 ng/ml. Preliminary dosages on plasma from leukemic patients treated with a 4 hr infusion of 75 mg/m2 daunorubicin either as free drug or complexed with DNA were reported. The results suggested a slower disposition of the daunorubicin-DNA complex.
描述了一种高效液相色谱法，用于在人血浆中低浓度下同时定量测定柔红霉素及其活性代谢物柔红霉醇。该方法的优点是特异性、灵敏度、最小的分析前处理和小样本量。该方法对血浆中低至10 ng/ml的柔红霉素和柔红霉醇水平敏感。报告了对白血病患者血浆的初步剂量测定结果，这些患者接受了4小时输注75 mg/m2柔红霉素，药物形式为游离药物或与DNA复合。结果表明柔红霉素-DNA复合物的处置较慢。
