Pinson A, Tirosh R
Laboratory for Myocardial Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Mol Cell Biochem. 1996 Jul-Aug;160-161:137-41. doi: 10.1007/BF00240043.
The LDH release pattern from cardiomyocytes under 'ischemia-like' conditions shows two phases. In the initial slow phase, reoxygenation immediately stops further enzyme release. Accelerated LDH release, which occurs concomitantly with Iysosomal enzyme release, characterizes the second phase of 'ischemia.' Reoxygenation at this stage does not put a stop to further enzyme release. Reoxygenation during the first phase of 'ischemia' rapidly restored ATP level, while in the second phase, ATP levels remained low even after 6 h of reoxygenation. This study as well as previous data seem to suggest that irreversible cellular damage leading to cell death, occurs by synergistic action of many effectors, each of which does not necessarily cause irreversible damage.
在“类缺血”条件下心肌细胞的乳酸脱氢酶（LDH）释放模式呈现两个阶段。在初始的缓慢阶段，再灌注会立即停止进一步的酶释放。加速的LDH释放，与溶酶体酶释放同时发生，是“缺血”第二阶段的特征。在此阶段再灌注并不能阻止进一步的酶释放。“缺血”第一阶段的再灌注迅速恢复了三磷酸腺苷（ATP）水平，而在第二阶段，即使再灌注6小时后ATP水平仍维持在低水平。这项研究以及先前的数据似乎表明，导致细胞死亡的不可逆细胞损伤是由许多效应器的协同作用引起的，其中每个效应器不一定会造成不可逆损伤。
