Whalen R G
Centre National de la Recherche Scientifique, Paris, France.
Emerg Infect Dis. 1996 Jul-Sep;2(3):168-75. doi: 10.3201/eid0203.960302.
A novel and powerful method for vaccine research, colloquially known as DNA vaccines, involves the deliberate introduction into tissues of a DNA plasmid carrying an antigen-coding gene that transfects cells in vivo and results in an immune response. DNA vaccines have several distinct advantages, which include ease of manipulation, use of a generic technology, simplicity of manufacture, and chemical and biological stability. In addition, DNA vaccines are a great leveler among re-searchers around the world because they provide unprecedented ease of experi-mentation. To facilitate diffusion of information, an Internet site has been established called THE DNA VACCINE WEB (URL:http://www.genweb.com/dnavax/dnavax.html). In this review, a brief survey is undertaken of the experimental models and preclinical work on DNA vaccines to contribute to a greater awareness of the possibilities for emerging infectious diseases.
一种新颖且强大的疫苗研究方法，通俗地称为DNA疫苗，它涉及将携带抗原编码基因的DNA质粒特意引入组织中，该质粒在体内转染细胞并引发免疫反应。DNA疫苗具有几个明显的优点，包括易于操作、采用通用技术、生产简单以及化学和生物学稳定性。此外，DNA疫苗在全球研究人员中是一个极大的平等器，因为它们提供了前所未有的实验便利性。为促进信息传播，已建立了一个名为“DNA疫苗网站”的互联网网站（网址：http://www.genweb.com/dnavax/dnavax.html）。在本综述中，对DNA疫苗的实验模型和临床前工作进行了简要概述，以提高对新兴传染病可能性的认识。
