Ilg E C, Schäfer B W, Heizmann C W
Division of Clinical Chemistry, Department of Pediatrics, University of Zurich, Switzerland.
Int J Cancer. 1996 Nov 4;68(3):325-32. doi: 10.1002/(SICI)1097-0215(19961104)68:3<325::AID-IJC10>3.0.CO;2-7.
The S100 Ca(2+)-binding proteins recently became of major interest because of their differential expression in neoplastic tissues, their involvement in metastatic processes, and the clustered organization of at least 10 S100 genes on human chromosome 1q21, a region frequently rearranged in several tumors. As a first attempt towards a specific and differentiated immunohistochemical classification of human tumors, we produced, purified and characterized a number of human recombinant S100 proteins and raised specific polyclonal antibodies. Their distinct cellular and intracellular localization was examined by immunohistochemical methods in normal and cancerogenic human tissues and cell lines. S100A1 and S100A2 can be detected in a few normal tissues only, whereas S100A4, S100A6, and S100B are expressed at higher levels in cancer tissues. In the future, these S100 antibodies will potentially be of great value in cancer diagnosis and therapy.
S100钙结合蛋白最近备受关注，原因在于它们在肿瘤组织中的差异表达、参与转移过程，以及人类染色体1q21上至少10个S100基因的成簇组织，该区域在多种肿瘤中经常发生重排。作为对人类肿瘤进行特异性和差异化免疫组织化学分类的首次尝试，我们制备、纯化并鉴定了多种人重组S100蛋白，并制备了特异性多克隆抗体。通过免疫组织化学方法在正常和致癌性人类组织及细胞系中检测了它们独特的细胞和细胞内定位。S100A1和S100A2仅在少数正常组织中可检测到，而S100A4、S100A6和S100B在癌组织中表达水平较高。未来，这些S100抗体在癌症诊断和治疗中可能具有巨大价值。
