Shrestha P, Muramatsu Y, Kudeken W, Mori M, Takai Y, Ilg E C, Schafer B W, Heizmann C W
Department of Oral and Maxillofacial Surgery, Asahi University School of Dentistry, Gifu, Japan.
Virchows Arch. 1998 Jan;432(1):53-9. doi: 10.1007/s004280050134.
The Ca(2+)-binding proteins S100A1, S100A2, S100A4, S100A6 and S100B were evaluated immunohistochemically in normal skin and skin appendage tumours. Epidermal basal cells, epithelial cells of sebaceous glands, hair follicle sheet epithelia and eccrine duct reacted strongly with an antiserum against human S100A2 but were nonreactive or weakly reactive to S100A1, S100A4, S100A6 and S100B. Varying types of skin appendage tumours and most peripheral cells in tumour nests of basal cell carcinoma and squamous cell carcinoma showed positive S100A2 immunoreactivity in neoplastic cells corresponding to basal cells but were nonreactive or faintly reactive for other S100 proteins. Langerhan's cells and melanocytes were labelled by S100B. Basophilic cells of calcifying epithelioma were occasionally stained with S100A2 antiserum. Eccrine poroma did not react with any S100 antiserum. Mixed tumours of the skin containing neoplastic myoepithelial cells stained strongly for S100A2 and S100B but only faintly for S100A1, S100A4, S100A6. This is the first report on selective evaluation of different S100 proteins in normal skin. These antibodies are valuable tools for better characterization of skin appendage tumours.
采用免疫组织化学方法对正常皮肤及皮肤附属器肿瘤中的钙结合蛋白S100A1、S100A2、S100A4、S100A6和S100B进行了评估。表皮基底细胞、皮脂腺上皮细胞、毛囊板层上皮和小汗腺导管与抗人S100A2抗血清反应强烈，但对S100A1、S100A4、S100A6和S100B无反应或反应较弱。不同类型的皮肤附属器肿瘤以及基底细胞癌和鳞状细胞癌肿瘤巢中的大多数外周细胞，在与基底细胞相对应的肿瘤细胞中显示出S100A2免疫反应阳性，但对其他S100蛋白无反应或反应微弱。朗格汉斯细胞和黑素细胞被S100B标记。钙化上皮瘤的嗜碱性细胞偶尔被S100A2抗血清染色。小汗腺汗孔瘤与任何S100抗血清均无反应。含有肿瘤性肌上皮细胞的皮肤混合瘤对S100A2和S100B染色强烈，但对S100A1、S100A4、S100A6仅染色微弱。这是关于正常皮肤中不同S100蛋白选择性评估的首次报道。这些抗体是更好地鉴定皮肤附属器肿瘤的有价值工具。
