Davin-Regli A, Bollet C, Auffray J P, Saux P, De Micco P
Laboratoire d'Hygiene et de Microbiologie, Hopital Salvator, Marseille, France.
J Hosp Infect. 1996 Jan;32(1):39-50. doi: 10.1016/s0195-6701(96)90163-2.
We used the technique of random amplification of polymorphic DNA (RAPD) to type 130 isolates of Stenotrophomonas (Xanthomonas) maltophilia, using four arbitrary short primers. Of the 130 isolates, 51 were from the hospital environment, 48 from clinical specimens and 31 were geographically diverse environmental isolates. DNA amplification with the four sets of primers generated 112 RAPD patterns that differed by two or more bands in one of the four primers. Sixteen pairs of isolates were of the same RAPD pattern and some of these pairs represented clinical strains obtained from patients hospitalized at the same time in the same ward. In three patients, two to three strains of S. maltophilia which gave different RAPD fingerprints were isolated on the same day from different specimens. RAPD fingerprinting demonstrated great genomic diversity within the species S. maltophilia and provided an effective method for the study of the epidemiology of both clinical and environmental strains.
我们使用多态性DNA随机扩增（RAPD）技术，用四条任意短引物对130株嗜麦芽窄食单胞菌（嗜麦芽黄单胞菌）进行分型。在这130株分离株中，51株来自医院环境，48株来自临床标本，31株是来自不同地理位置的环境分离株。用这四组引物进行DNA扩增产生了112种RAPD图谱，这些图谱在四条引物中的一条上有两条或更多条带的差异。16对分离株具有相同的RAPD图谱，其中一些对代表了在同一病房同时住院的患者所获得的临床菌株。在三名患者中，同一天从不同标本中分离出两到三株产生不同RAPD指纹图谱的嗜麦芽窄食单胞菌菌株。RAPD指纹分析显示嗜麦芽窄食单胞菌物种内具有高度的基因组多样性，并为临床和环境菌株的流行病学研究提供了一种有效的方法。
