Eriks I S, Munck K T, Besser T E, Cantor G H, Kapur V
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman 99164-7040, USA.
J Clin Microbiol. 1996 Mar;34(3):734-7. doi: 10.1128/jcm.34.3.734-737.1996.
Mycobacterium avium subsp. avium (M. avium) and M. avium subsp. paratuberculosis (M. paratuberculosis), intracellular bacteria that can cause chronic granulomatous enteritis in cattle, are difficult to distinguish on the basis of growth and biochemical characteristics. We report the development of a PCR-based strategy for the rapid differentiation of isolates of M. avium from isolates of M. paratuberculosis. Restriction fragment length polymorphism was identified by PCR amplification and subsequent restriction enzyme digestion with PstI of a 960-bp fragment of the 65-kDa heat shock protein (hsp65) from 21 clinical isolates of M. paratuberculosis and 14 isolates of M. avium. These results indicate that a restriction fragment length polymorphism in the hsp65 gene can be used for the rapid differentiation of clinical isolates of M. paratuberculosis and M. avium.
鸟分枝杆菌鸟亚种（M. avium）和副结核分枝杆菌（M. paratuberculosis）是可引起牛慢性肉芽肿性肠炎的胞内细菌，根据生长和生化特征难以区分。我们报告了一种基于聚合酶链反应（PCR）的策略，用于从副结核分枝杆菌分离株中快速鉴别鸟分枝杆菌分离株。通过PCR扩增以及随后用PstI对来自21株副结核分枝杆菌临床分离株和14株鸟分枝杆菌分离株的65-kDa热休克蛋白（hsp65）的960-bp片段进行限制性酶切，鉴定出限制性片段长度多态性。这些结果表明，hsp65基因中的限制性片段长度多态性可用于副结核分枝杆菌和鸟分枝杆菌临床分离株的快速鉴别。
