Caicedo A, d'Aldin C, Puel J L, Eybalin M
INSERM U. 254, Laboratoire de Neurobiologie de l'Audition, CHU Hôpital St. Charles, Montpellier, France.
Anat Embryol (Berl). 1996 Nov;194(5):465-87. doi: 10.1007/BF00185994.
This study was intended to provide an overview of the distribution of calcium-binding proteins in the rodent auditory brainstem. We based our observations on immunohistochemical material obtained in the guinea pig, a species widely used in auditory research in which a mapping of calcium-binding proteins in the auditory brainstem is still missing. Differences in the amounts of these proteins throughout the auditory brainstem were further analyzed semiquantitatively. Parvalbumin was present in most neurons and their axon terminals throughout the ascending auditory brainstem. Nuclei that surround the main relay nuclei of the ascending auditory pathway lacked labeling. Calretinin staining was prominent in spherical and globular cells of the cochlear nucleus, in their axon terminals in the superior olivary complex, and in principal cells of the medial superior olive. Measures of optical densities showed that auditory neurons involved in sound localization had the highest calretinin labeling levels. Calbindin D-28k was present in cartwheel cells of the dorsal cochlear nucleus, in almost all neurons of the medial nucleus of the trapezoid body, and in globular cells in the ventral nucleus of the lateral lemniscus. The labeling patterns for calretinin and calbindin D-28k were non-overlapping throughout the auditory brainstem. This was also evident in the ventral nucleus of the lateral lemniscus where calbindin D-28k-immunoreactive terminals were found in the medial portion, while the calretinin-immunoreactive terminals were observed in the lateral portion. This study presents the first direct and comprehensive comparison of these three calcium-binding proteins in the auditory brainstem of a rodent. Each antibody yields a unique staining pattern that provides a basis for further defining neuronal populations. In addition, since their axons are also selectively stained, auditory nuclei can further be compartmentalized based on different terminal fields. These immunoreactivities have provided clues to the complex structure of the auditory brainstem.
本研究旨在概述啮齿动物听觉脑干中钙结合蛋白的分布情况。我们的观察基于在豚鼠身上获得的免疫组化材料，豚鼠是听觉研究中广泛使用的一种物种，目前仍缺乏对其听觉脑干中钙结合蛋白的图谱研究。我们进一步对这些蛋白在整个听觉脑干中的含量差异进行了半定量分析。小白蛋白存在于整个听觉脑干上行通路的大多数神经元及其轴突终末中。围绕听觉上行通路主要中继核的核团未被标记。钙视网膜蛋白染色在耳蜗核的球状和球囊状细胞、它们在上橄榄复合体中的轴突终末以及内侧上橄榄核的主细胞中较为突出。光密度测量结果显示，参与声音定位的听觉神经元的钙视网膜蛋白标记水平最高。钙结合蛋白D - 28k存在于背侧耳蜗核的车轮状细胞、斜方体内侧核的几乎所有神经元以及外侧丘系腹侧核的球囊状细胞中。在整个听觉脑干中，钙视网膜蛋白和钙结合蛋白D - 28k的标记模式并不重叠。这在外侧丘系腹侧核中也很明显，在该核的内侧部分发现了钙结合蛋白D - 28k免疫反应性终末，而在外侧部分观察到了钙视网膜蛋白免疫反应性终末。本研究首次对啮齿动物听觉脑干中的这三种钙结合蛋白进行了直接而全面的比较。每种抗体都产生独特的染色模式，为进一步界定神经元群体提供了依据。此外，由于它们的轴突也被选择性染色，因此可以根据不同的终末场进一步划分听觉核团。这些免疫反应性为听觉脑干的复杂结构提供了线索。
