大肠杆菌K-12连锁图谱，第10版：物理图谱。
Linkage map of Escherichia coli K-12, edition 10: the physical map.
作者信息
Rudd K E
机构信息
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, Florida 33101-6129, USA.
出版信息
Microbiol Mol Biol Rev. 1998 Sep;62(3):985-1019. doi: 10.1128/MMBR.62.3.985-1019.1998.
Abstract
A physical map, EcoMap10, of the now completely sequenced Escherichia coli chromosome is presented. Calculated genomic positions for the eight restriction enzymes BamHI, HindIII, EcoRI, EcoRV, BglI, KpnI, PstI, and PvuII are depicted. Both sequenced and unsequenced Kohara/Isono miniset clones are aligned to this calculated restriction map. DNA sequence searches identify the precise locations of insertion sequence elements and repetitive extragenic palindrome clusters. EcoGene10, a revised set of genes and functionally uncharacterized open reading frames (ORFs), is also depicted on EcoMap10. The complete set of unnamed ORFs in EcoGene10 are assigned provisional names beginning with the letter "y" by using a systematic nomenclature.
摘要
本文展示了现已完成全序列测定的大肠杆菌染色体的物理图谱EcoMap10。描绘了八种限制酶BamHI、HindIII、EcoRI、EcoRV、BglI、KpnI、PstI和PvuII的计算基因组位置。已测序和未测序的小原/矶野微型克隆集与该计算的限制酶图谱进行了比对。DNA序列搜索确定了插入序列元件和重复的基因外回文簇的精确位置。EcoGene10，一组经过修订的基因和功能未表征的开放阅读框（ORF），也展示在EcoMap10上。EcoGene10中完整的未命名ORF集通过系统命名法被赋予了以字母“y”开头的临时名称。
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