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通过自由区电泳揭示人前列腺小体与小鼠精子的黏附及其被单克隆抗体的置换。

Adherence of human prostasomes to mouse spermatozoa and their displacement by monoclonal antibodies as revealed by free zone electrophoresis.

作者信息

Ronquist G, Fabiani R, Jin M, Nilsson B O, Elenbring K, Hjerten S

机构信息

Department of Clinical Chemistry, University Hospital, University of Uppsala, Sweden.

出版信息

Arch Androl. 1996 Mar-Apr;36(2):101-7. doi: 10.3109/01485019608987085.

DOI:10.3109/01485019608987085
PMID:8907670
Abstract

Prostasomes, small corpuscular organelles derived from the prostate gland, were isolated from human seminal plasma by means of ultracentrifugation and Sephadex G 200 chromatography to examine their interactive ability with mouse spermatozoa as assessed by free zone electrophoresis. During the run, human prostasomes and mouse spermatozoa approached each other and finally fused into one single peak that was not further dissociated. In some cases the fusion of prostasomes and spermatozoa failed to appear following pretreatment of prostasomes with monoclonal antibodies raised against these organelles.

摘要

前列腺小体是源自前列腺的微小颗粒细胞器,通过超速离心和葡聚糖凝胶G 200色谱法从人类精浆中分离出来,以通过自由区电泳评估其与小鼠精子的相互作用能力。在电泳过程中,人类前列腺小体和小鼠精子相互靠近,最终融合成一个不再分离的单一峰。在某些情况下,用针对这些细胞器产生的单克隆抗体对前列腺小体进行预处理后,前列腺小体与精子未能出现融合现象。

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Adherence of human prostasomes to mouse spermatozoa and their displacement by monoclonal antibodies as revealed by free zone electrophoresis.通过自由区电泳揭示人前列腺小体与小鼠精子的黏附及其被单克隆抗体的置换。
Arch Androl. 1996 Mar-Apr;36(2):101-7. doi: 10.3109/01485019608987085.
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