Fabiani R, Johansson L, Lundkvist O, Ronquist G
Department of Clinical Chemistry, University Hospital, University of Uppsala, Sweden.
Hum Reprod. 1994 Aug;9(8):1485-9. doi: 10.1093/oxfordjournals.humrep.a138735.
Prostasomes, which are prostate-derived organelles, were purified from human seminal plasma for inclusion in Earle's balanced salt solution (EBSS) medium with or without human serum albumin. These media were used for swim-up experiments and the subsequent analyses of sperm motility parameters at different incubation times. The yield of motile spermatozoa after swim-up in EBSS medium enriched with boiled prostasomes was increased by 32% compared with EBSS containing albumin. Native prostasomes were less active. Combinations of albumin and either prostasomes or boiled prostasomes significantly increased the recovery of motile spermatozoa and also increased the percentage of spermatozoa displaying progressive motility after 1 h of incubation. Media lacking albumin showed lower values regarding progressive motility after 22 h of incubation. A beneficial effect of prostasomes was noted on lateral head displacement and percentage of hyperactive spermatozoa during the first 6 h of incubation. These results suggest that inclusion of prostasomes, especially boiled prostasomes, in swim-up media may improve the recovery of hyperactive motile spermatozoa for up to 6 h in cases of established male factor infertility, and consequently increase the opportunities for fertilization.
前列腺小体是源自前列腺的细胞器，从人精浆中纯化出来，加入或不加入人血清白蛋白，置于Earle平衡盐溶液（EBSS）培养基中。这些培养基用于上游法实验以及在不同孵育时间对精子活力参数进行后续分析。与含白蛋白的EBSS相比，在富含煮沸前列腺小体的EBSS培养基中进行上游法后，活动精子的产量增加了32%。天然前列腺小体的活性较低。白蛋白与前列腺小体或煮沸前列腺小体的组合显著提高了活动精子的回收率，并且在孵育1小时后，显示进行性运动的精子百分比也有所增加。缺乏白蛋白的培养基在孵育22小时后，进行性运动的值较低。在孵育的前6小时内，观察到前列腺小体对头部横向位移和活跃精子百分比有有益影响。这些结果表明，在男性因素不育确诊的情况下，在上游法培养基中加入前列腺小体，尤其是煮沸的前列腺小体，可能会在长达6小时内提高活跃运动精子的回收率，从而增加受精机会。
