Assarian G S, Oberleas D
Clin Chem. 1977 Sep;23(9):1771-2.
A pooled sample of hair was divided and portions prepared for analysis by three washing procedures, to evaluate the effect of washing procedure on the subsequent trace-element (Zn, Cu, Mg) content. The methods selected were a detergent wash, a hexane-ethanol wash, and an acetone-ether-detergent wash. For all elements, there was a significant difference among the results after these wash procedures. Magnesium content of hair was most affected by washing, containing less than half of the magnesium of the unwashed hair. The detergent wash removed the most zinc and magnesium; the acetone-ether-detergent wash removed the most copper. Our results indicate that the trace-element analysis of hair is sensitive to the preparation technique and therefore is an unreliable source of information about trace-element status.
采集的毛发混合样本被分成若干部分，并通过三种清洗程序进行处理以用于分析，目的是评估清洗程序对后续微量元素（锌、铜、镁）含量的影响。所选用的方法分别是用洗涤剂清洗、用己烷 - 乙醇清洗以及用丙酮 - 乙醚 - 洗涤剂清洗。对于所有元素而言，这些清洗程序后的结果存在显著差异。毛发中的镁含量受清洗影响最大，清洗后毛发中的镁含量不到未清洗毛发的一半。用洗涤剂清洗去除的锌和镁最多；用丙酮 - 乙醚 - 洗涤剂清洗去除的铜最多。我们的结果表明，毛发的微量元素分析对制备技术很敏感，因此是关于微量元素状态的不可靠信息来源。
