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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Korkotian E, Segal M

Department of Neurobiology, Weizmann Institute, Rehovot, Israel.

J Physiol. 1996 Oct 1;496 ( Pt 1)(Pt 1):39-48. doi: 10.1113/jphysiol.1996.sp021663.

Changes in free intracellular calcium concentrations ([Ca2+]i) were measured in the nucleus and perinuclear regions of cultured rat hippocampal neurons using either fura-2 or fluo-3 calcium indicators. 2. Brief application of glutamate caused a transient rise of [Ca2+]i in all cell compartments, which recovered to pre-drug levels in all but the nuclear region. The new, higher level of nuclear calcium ([Ca2+]n) was sustained for as long as the cell was monitored. 3. The new level of [Ca2+]n was dependent on the magnitude of the calcium transient, and was higher in older cells in culture, but it did not affect responses to subsequent applications of glutamate. 4. The sustained elevation of [Ca2+]n was prevented by drugs which affect calcium stores (caffeine, ryanodine and Ruthenium Red), indicating that an extranuclear calcium store interacts with [Ca2+]n.

使用fura - 2或fluo - 3钙指示剂，在培养的大鼠海马神经元的细胞核和核周区域测量细胞内游离钙浓度（[Ca2+]i）的变化。2. 短暂施加谷氨酸会导致所有细胞区室中的[Ca2+]i瞬时升高，除了核区域外，其他区域的[Ca2+]i会恢复到药物处理前的水平。只要对细胞进行监测，细胞核钙（[Ca2+]n）的新的较高水平就会持续存在。3. [Ca2+]n的新水平取决于钙瞬变的幅度，并且在培养的较老细胞中更高，但它不影响对随后施加谷氨酸的反应。4. 影响钙储存的药物（咖啡因、ryanodine和钌红）可阻止[Ca2+]n的持续升高，这表明核外钙储存与[Ca2+]n相互作用。

Korkotian E, Segal M

Department of Neurobiology, Weizmann Institute, Rehovot, Israel.

J Physiol. 1996 Oct 1;496 ( Pt 1)(Pt 1):39-48. doi: 10.1113/jphysiol.1996.sp021663.

Changes in free intracellular calcium concentrations ([Ca2+]i) were measured in the nucleus and perinuclear regions of cultured rat hippocampal neurons using either fura-2 or fluo-3 calcium indicators. 2. Brief application of glutamate caused a transient rise of [Ca2+]i in all cell compartments, which recovered to pre-drug levels in all but the nuclear region. The new, higher level of nuclear calcium ([Ca2+]n) was sustained for as long as the cell was monitored. 3. The new level of [Ca2+]n was dependent on the magnitude of the calcium transient, and was higher in older cells in culture, but it did not affect responses to subsequent applications of glutamate. 4. The sustained elevation of [Ca2+]n was prevented by drugs which affect calcium stores (caffeine, ryanodine and Ruthenium Red), indicating that an extranuclear calcium store interacts with [Ca2+]n.

使用fura - 2或fluo - 3钙指示剂，在培养的大鼠海马神经元的细胞核和核周区域测量细胞内游离钙浓度（[Ca2+]i）的变化。2. 短暂施加谷氨酸会导致所有细胞区室中的[Ca2+]i瞬时升高，除了核区域外，其他区域的[Ca2+]i会恢复到药物处理前的水平。只要对细胞进行监测，细胞核钙（[Ca2+]n）的新的较高水平就会持续存在。3. [Ca2+]n的新水平取决于钙瞬变的幅度，并且在培养的较老细胞中更高，但它不影响对随后施加谷氨酸的反应。4. 影响钙储存的药物（咖啡因、ryanodine和钌红）可阻止[Ca2+]n的持续升高，这表明核外钙储存与[Ca2+]n相互作用。