一种用于定量评估重症肌无力患者抗人乙酰胆碱受体抗体的放射免疫测定法。
A radioimmunoassay for the quantitative evaluation of anti-human acetylcholine receptor antibodies in myasthenia gravis.
作者信息
Monnier V M, Fulpius B W
出版信息
Clin Exp Immunol. 1977 Jul;29(1):16-22.
Abstract
A radioimmunoassay was developed for the quantitative evaluation of antibodies to the acetylcholine receptor in the serum of myasthenic patients. AcChR was extracted from human muscle. A detailed preparation of the 125I-labelled alpha-Bgt-AcChR complex used as antigen is reported. Usually, an average of 20 pmol were obtained form 100 g muscle. This preparation is stable for 1 month in presence of an inhibitor of proteolysis and sufficient for performing about fifteen assays. The labelled complex was incubated with increasing amounts of sera and precipitated with anti-human IgG serum. Titres were expressed in pmol 125I-labelled alpha-Bgt-AcChR complex precipitated per ml serum. Out of thirty-nine sera tested thirty-six had positive titres ranging from 0-1-46 pmol/ml. No anti-AcChR were detected in the sera from twenty-seven patients used as controls.
摘要
已开发出一种放射免疫分析法，用于定量评估重症肌无力患者血清中抗乙酰胆碱受体抗体。乙酰胆碱受体从人体肌肉中提取。报告了用作抗原的125I标记的α-银环蛇毒素-乙酰胆碱受体复合物的详细制备方法。通常，从100克肌肉中平均可获得20皮摩尔。该制剂在存在蛋白水解抑制剂的情况下可稳定保存1个月，足以进行约15次检测。将标记的复合物与越来越多的血清孵育，并用抗人IgG血清沉淀。滴度以每毫升血清中沉淀的125I标记的α-银环蛇毒素-乙酰胆碱受体复合物的皮摩尔数表示。在检测的39份血清中，36份的滴度为阳性，范围为0.1至46皮摩尔/毫升。在用作对照的27名患者的血清中未检测到抗乙酰胆碱受体。
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