莱氏无胆甾原体对小鼠和人类淋巴细胞培养的影响。
Effect of A. laidlawii on murine and human lymphocyte cultures.
作者信息
Kirchner H, Brunner H, Rühl H
出版信息
Clin Exp Immunol. 1977 Jul;29(1):176-80.
Heat-inactivated A. laidlawii (AL) was found to be a potent mitogen for mouse spleen cells. Spleen cells from homozygous nude mice and spleen cells treated with anti-theta serum and complement responded as well as their respective controls, indicating that AL represented a B-cell mitogen for mouse spleen cells. Spleen cells from LPS-unresponsive C3H/Hej mice responded well to AL. The peripheral blood leucocytes from unselected human donors were also stimulated by AL, which appeared to represent a T-cell mitogen for human leucocytes. However, the possibility that it acted as a specific antigen could not be excluded. Attention was drawn to the possibility that the presence of mycoplasma might considerably affect the results of tests where tissue-culture cells or derivatives thereof are added to leucocyte cultures.
热灭活的莱氏无胆甾原体（AL）被发现是小鼠脾细胞的一种强效促细胞分裂剂。纯合裸鼠的脾细胞以及用抗θ血清和补体处理过的脾细胞，其反应与各自的对照相同，这表明AL是小鼠脾细胞的一种B细胞促细胞分裂剂。对脂多糖无反应的C3H/Hej小鼠的脾细胞对AL反应良好。未经过筛选的人类供体的外周血白细胞也受到AL的刺激，这似乎表明AL是人类白细胞的一种T细胞促细胞分裂剂。然而，不能排除它作为一种特异性抗原起作用的可能性。有人提醒注意，支原体的存在可能会极大地影响将组织培养细胞或其衍生物添加到白细胞培养物中的测试结果。
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