紫花苜蓿中三萜类糖苷的化学性质与生物活性

Chemistry and biological activity of triterpenoid glycosides from Medicago sativa.

作者信息

Timbekova A E, Isaev M I, Abubakirov N K

机构信息

Institute of Chemistry of Plant Substances, Uzbek AS, Tashkent, Uzbekistan.

出版信息

Adv Exp Med Biol. 1996;405:171-82. doi: 10.1007/978-1-4613-0413-5_14.

DOI:10.1007/978-1-4613-0413-5_14

PMID:8910703

Abstract

Ten triterpenoid glycosides have been isolated from the roots of Medicago sativa L. (Leguminosae) including eight new glycosides and two previously known, medicagenic acid 3-O-beta-D-glucopyranoside and hederagenin 3-O-[beta-D-glucopyranosyl(1-->2)-alpha-L-arabinopyranoside]. Four new saponins were identified as glycosides of medicagenic acid, four from hederagenin. Their structures were elucidated by a combination of chemical and spectroscopic techniques (IR, EI/MS, NMR 1H and 13C). In some cases we used fast atom bombardment mass spectra (FAB/MS), NMR 1H difference spectra (homonuclear double resonance, Overhauser effect), NMR 13C partially decoupled spectra (off resonance), and two-dimensional 1H-1H correlating spectra (COSY 1H-1H). The inhibitory effects of these glycosides on growth and seed germinations and on some phytopathogenic bacteria have also been tested.

摘要

从紫花苜蓿（豆科）根部分离出了十种三萜类糖苷，其中包括八种新糖苷以及两种已知的苜蓿酸3 - O - β - D - 吡喃葡萄糖苷和常春藤皂苷元3 - O - [β - D - 吡喃葡萄糖基(1→2)-α - L - 阿拉伯吡喃糖苷]。四种新皂苷被鉴定为苜蓿酸的糖苷，另外四种是常春藤皂苷元的糖苷。通过化学和光谱技术（红外光谱、电子轰击质谱、核磁共振氢谱和碳谱）相结合的方法阐明了它们的结构。在某些情况下，我们使用了快原子轰击质谱（FAB/MS）、核磁共振氢谱差异谱（同核双共振、奥弗豪泽效应）、核磁共振碳谱部分去耦谱（偏共振）以及二维氢 - 氢相关谱（COSY 1H - 1H）。还测试了这些糖苷对生长、种子萌发以及一些植物病原菌的抑制作用。