从具有神经保护活性的紫花苜蓿中分离三萜皂苷。
Isolation of triterpenoid saponins from Medicago sativa L. with neuroprotective activities.
机构信息
Institute of Functional Molecules, Liaoning Province Key Laboratory of Green Functional Molecular Design and Development, Shenyang Key Laboratory of Targeted Pesticides, Shenyang University of Chemical Technology, Shenyang 110142, Liaoning, PR China.
Institute of Functional Molecules, Liaoning Province Key Laboratory of Green Functional Molecular Design and Development, Shenyang Key Laboratory of Targeted Pesticides, Shenyang University of Chemical Technology, Shenyang 110142, Liaoning, PR China; College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang 110142, Liaoning, PR China.
出版信息
Bioorg Med Chem Lett. 2020 Feb 15;30(4):126956. doi: 10.1016/j.bmcl.2020.126956. Epub 2020 Jan 7.
Three new pentacyclic triterpenoid saponins (1-3), together with medicagenic acid (4) were isolated and purified from 70% EtOH extract of Medicago sativa L. by different column chromatographic and semi-preparative HPLC. Their structures were established by direct interpretation of their spectral data, mainly HR-ESI-MS, 1D-NMR, 2D-NMR, and chemical methods, as well as comparison with literature data. Additionally, all isolates were evaluated for their neuroprotective activities against HO-induced damage in human neuroblastoma SHSY5Y cells. As a results, compounds 1 and 2 (67.14% and 73.05%) exhibited potent neuroprotective activities. These findings provide new insights into developing better treatment of neurodegenerative diseases for M. sativa in the future.
从紫花苜蓿 70%乙醇提取物中，通过不同的柱色谱和半制备 HPLC 分离和纯化了三种新的五环三萜皂苷（1-3）和羽扇豆酸（4）。通过直接解析其光谱数据，主要是 HR-ESI-MS、1D-NMR、2D-NMR 和化学方法，并与文献数据进行比较，确定了它们的结构。此外，还评估了所有分离物对 HO 诱导的人神经母细胞瘤 SHSY5Y 细胞损伤的神经保护活性。结果表明，化合物 1 和 2（67.14%和 73.05%）表现出很强的神经保护活性。这些发现为今后利用紫花苜蓿开发治疗神经退行性疾病的更好方法提供了新的思路。
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