Kincaid A E, Wilson C J
Department of Anatomy and Neurobiology, College of Medicine, University of Tennessee, Memphis 38163, USA.
J Comp Neurol. 1996 Oct 28;374(4):578-92. doi: 10.1002/(SICI)1096-9861(19961028)374:4<578::AID-CNE7>3.0.CO;2-Z.
The distribution of rat corticostrial axons in the patch (striosome) and matrix compartments of the neostriatum was studied by using axonal labeling with biotinylated dextran amine (BDA) and identifying patch and matrix in the same section with calbindin immunocytochemistry. Small injections of BDA were made in the anterior cingulate, medial agranular, lateral agranular, or somatosensory cortex. Each area projected to both the patch and matrix compartments, except for the somatosensory cortex, which had only matrix projections. Within the remaining cortical areas, injections in layers Vb and VI preferentially labeled axons in patches whereas injections in layers III-Va preferentially labeled matrix axons. Axons from these injections formed varicosities preferentially, but not exclusively, in one compartment. There was a population of axons that crossed compartmental boundaries and arborized in both patch and matrix. Two distinct patterns of corticostriatal axonal arborizations were observed. Small, discrete foci of innervation were seen in the patch compartment and in some regions of the matrix. The focal arborizations in the matrix were observed through the rostrocaudal extent of the neostriatum but were most obvious in the caudal one-third. They resembled the matrisomes observed in cat and primate corticostriatal projections. The second pattern of innervation consisted of extended axonal arborizations that covered large regions of the rostral neostriatal matrix. These results support the concept of multiple classes of corticostriatal neurons having different targets within the neostriatum, following different topographical rules, and having different but overlapping distributions across cortical areas.
采用生物素化葡聚糖胺（BDA）进行轴突标记，并在同一切片中用钙结合蛋白免疫细胞化学鉴定斑块和基质，研究了大鼠皮质纹状体轴突在新纹状体的斑块（纹状体小体）和基质区室中的分布。在前扣带回、内侧无颗粒、外侧无颗粒或体感皮层进行小剂量BDA注射。除体感皮层仅投射到基质区室外，每个区域都投射到斑块和基质区室。在其余皮质区域内，Vb层和VI层的注射优先标记斑块中的轴突，而III - Va层的注射优先标记基质轴突。这些注射产生的轴突优先在一个区室形成曲张，但并非唯一。有一群轴突穿过区室边界并在斑块和基质中都形成分支。观察到两种不同的皮质纹状体轴突分支模式。在斑块区室和基质的一些区域可见小的、离散的神经支配灶。基质中的局灶性分支在新纹状体的前后范围内都有观察到，但在尾侧三分之一处最为明显。它们类似于在猫和灵长类动物皮质纹状体投射中观察到的基质小体。第二种神经支配模式由延伸的轴突分支组成，覆盖了纹状体前部基质的大片区域。这些结果支持了这样的概念，即多种类型的皮质纹状体神经元在新纹状体内有不同的靶点，遵循不同的拓扑规则，并且在不同皮质区域有不同但重叠的分布。
