间质性膀胱炎患者膀胱尿路上皮硫酸软骨素蛋白聚糖缺乏。
A deficit of chondroitin sulfate proteoglycans on the bladder uroepithelium in interstitial cystitis.
作者信息
Hurst R E, Roy J B, Min K W, Veltri R W, Marley G, Patton K, Shackelford D L, Stein P, Parsons C L
机构信息
Department of Urology, College of Medicine, Oklahoma University Health Sciences Center, Oklahoma City, USA.
出版信息
Urology. 1996 Nov;48(5):817-21. doi: 10.1016/S0090-4295(96)00322-6.
OBJECTIVES
To investigate the abundance of chondroitin sulfate proteoglycans at the bladder lumenal and subepithelial surfaces in bladder biopsies derived from patients with interstitial cystitis (IC) and controls.
METHODS
Tissue sections derived from biopsies from 31 IC patients and 24 pathologically normal control sections were labeled for proteoglycans using the 2B6 anti-"stub" antibody and detected by immunohistochemistry.
RESULTS
On the lumenal surface, 5 of 31 (19%) IC sections were positive for proteoglycans versus 14 of 24 (58%) control sections (P = 0.00011). At the basal surface, 5 of 19 IC patients were positive versus 7 of 12 controls (P = 0.032).
CONCLUSIONS
A deficit of bladder lumenal and basal proteoglycans is associated with IC. The deficit in basal layer proteoglycans suggests an altered urothelial differentiation program. The lumenal deficit suggests that the charge-dependent exclusion of ions from the bladder surface is compromised in IC.
目的
研究间质性膀胱炎（IC）患者及对照者膀胱活检组织中膀胱腔面和上皮下表面硫酸软骨素蛋白聚糖的含量。
方法
采用2B6抗“短肽”抗体对31例IC患者的活检组织切片和24例病理正常对照组织切片进行蛋白聚糖标记，并通过免疫组织化学法进行检测。
结果
在腔面，31例IC切片中有5例（19%）蛋白聚糖呈阳性，而24例对照切片中有14例（58%）呈阳性（P = 0.00011）。在基底表面，19例IC患者中有5例呈阳性，12例对照中有7例呈阳性（P = 0.032）。
结论
膀胱腔面和基底蛋白聚糖缺乏与IC有关。基底膜蛋白聚糖缺乏提示尿路上皮分化程序改变。腔面缺乏提示IC患者膀胱表面离子的电荷依赖性排斥受损。
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