Bourguet D, Capela R, Raymond M
Institut des Sciences de l'Evolution (UMR 5554), Université Montpellier II, France.
J Econ Entomol. 1996 Oct;89(5):1060-6. doi: 10.1093/jee/89.5.1060.
Resistance mechanisms of a strain (PRAIAS) of northern house mosquito, Culex pipiens L., collected in Portugal in 1993, and highly resistant to organophosphates and carbamates, were investigated by comparing the resistance characteristics to 3 organophosphorous (temephos, chlorpyrifos, malathion) and 1 carbamate (propoxur) insecticides in the presence or absence of synergists; and by determining the possible occurrence of overproduced esterases or insensitive acetylcholinesterase (AChE). The reference strain MSE from southern France, with an insensitive AChE, was included in all analyses for comparison. For organophosphorous insecticides, resistance in PRAIAS was caused by an insensitive AChE and an increase in oxidative metabolism, although the 2nd mechanism has only a marginal effect. For propoxur, the insensitive AChE was the only resistance mechanism detected. Biochemical properties of both the French and Portuguese insensitive AChEs were similar. We cannot exclude the possibility that PRAIAS and MSE strains possess exactly the same insensitive AChE allele.
1993年在葡萄牙采集的北方家蚊（库蚊）品系（PRAIAS）对有机磷和氨基甲酸酯类具有高度抗性，通过比较在有或无增效剂存在的情况下对3种有机磷（双硫磷、毒死蜱、马拉硫磷）和1种氨基甲酸酯（残杀威）杀虫剂的抗性特征，以及测定是否存在酯酶过量产生或不敏感的乙酰胆碱酯酶（AChE），对其抗性机制进行了研究。来自法国南部的具有不敏感AChE的参考品系MSE被纳入所有分析以作比较。对于有机磷杀虫剂，PRAIAS中的抗性是由不敏感的AChE和氧化代谢增加引起的，尽管第二种机制只有边际效应。对于残杀威，检测到的唯一抗性机制是不敏感的AChE。法国和葡萄牙不敏感AChE的生化特性相似。我们不能排除PRAIAS和MSE品系拥有完全相同的不敏感AChE等位基因的可能性。
