Takamura H, Takasaki K, Tsurumoto H, Kanda Y, Kobayashi T, Yoshimi R
Department of Otolaryngology, Nagasaki University School of Medicine.
Nihon Jibiinkoka Gakkai Kaiho. 1996 Sep;99(9):1172-5. doi: 10.3950/jibiinkoka.99.1172.
Nasal polyps, in general, have been histologically classified as edematous, adenocystic and fibrous types. It has recently been pointed out that eosinophilic infiltration in nasal polyps has increased along with an increase in nasal allergy. The purpose of this study was to classify the nasal polyps histologically and to examine the mode of infiltration and activation of eosinophils by means of immunohistochemistry. The subjects were 101 patients who underwent surgery for nasal polyps. There were 66 males and 35 females ranging in age from 12 to 76 years. Resected polyps were fixed in formalin, embedded in paraffin, and sliced at a thickness of 4 microns. Hematoxylin-eosin staining and streptavidin- biotinyl peroxidase complex staining were used to detect eosinophils. The results were as follows: 1) The nasal polyps were, histologically, edematous in 88.1%, adenocystic in 47.5%, and fibrous in 19.8%. 2) The number and distribution of EG1-positive cells and EG2-positive cells were almost the same in the superficial layer of the lamina propria. This study suggests that eosinophilic infiltration into nasal polyps occurs mainly in edematous polyps and that the majority of the infiltrating eosinophils are activated.
一般来说，鼻息肉在组织学上可分为水肿型、腺囊型和纤维型。最近有研究指出，随着鼻过敏的增加，鼻息肉中的嗜酸性粒细胞浸润也有所增加。本研究的目的是对鼻息肉进行组织学分类，并通过免疫组织化学方法研究嗜酸性粒细胞的浸润和激活方式。研究对象为101例接受鼻息肉手术的患者。其中男性66例，女性35例，年龄在12岁至76岁之间。切除的息肉用福尔马林固定，石蜡包埋，切成4微米厚的切片。采用苏木精-伊红染色和链霉亲和素-生物素过氧化物酶复合物染色检测嗜酸性粒细胞。结果如下：1）组织学上，鼻息肉水肿型占88.1%，腺囊型占47.5%，纤维型占19.8%。2）固有层浅层EG1阳性细胞和EG2阳性细胞的数量和分布基本相同。本研究表明，嗜酸性粒细胞浸润鼻息肉主要发生在水肿型息肉中，且大多数浸润的嗜酸性粒细胞被激活。
