Teramoto N, Cao L, Kawasaki N, Tonoyama Y, Sarker A B, Yoshino T, Takahashi K, Akagi T
Department of Pathology, Okayama University Medical School, Japan.
Acta Med Okayama. 1996 Oct;50(5):267-70. doi: 10.18926/AMO/30493.
Reed-Sternberg cells (RS cells) of Hodgkin's disease (HD) are frequently infected with Epstein-Barr virus (EBV) and express EBV-encoded nonpolyadenylated RNA transcripts (EBER)-1. EBV latency has been classified into three distinct forms: Latency I, expressing only one of the latent proteins, EBV nuclear antigen (EBNA)-1, latency II, coexpressing EBNA-1 and LMPs, and latency III, expressing all latent viral proteins. RS cells express LMP-1 in addition to EBNA-1 and are considered to be EBV latency II frequently encountered in nasopharyngeal carcinoma. We examined 13 cases of EBV-infected HD by combined EBER-1 in situ hybridization and immunostaining for LMP-1. All of the RS cells expressed EBER-1, but a substantial number of EBER-1+ RS, cells were negative for LMP-1. The percentage of LMP-1+ RS cells out of EBER-1+ RS cells varied from 7% to 100% (average 69%). In this study, we showed that all EBV-infected RS cells were not restricted to latency II, and some belonged to latency I.
霍奇金淋巴瘤（HD）的里德-施特恩伯格细胞（RS细胞）常感染爱泼斯坦-巴尔病毒（EBV）并表达EBV编码的非多聚腺苷酸化RNA转录本（EBER）-1。EBV潜伏感染已被分为三种不同形式：潜伏感染I型，仅表达一种潜伏蛋白，即EBV核抗原（EBNA）-1；潜伏感染II型，共表达EBNA-1和潜伏膜蛋白（LMPs）；潜伏感染III型，表达所有潜伏病毒蛋白。RS细胞除了表达EBNA-1外还表达LMP-1，被认为是鼻咽癌中常见的EBV潜伏感染II型。我们通过联合EBER-1原位杂交和LMP-1免疫染色检测了13例EBV感染的HD病例。所有RS细胞均表达EBER-1，但相当数量的EBER-1阳性RS细胞LMP-1呈阴性。LMP-1阳性RS细胞在EBER-1阳性RS细胞中所占的百分比从7%到100%不等（平均69%）。在本研究中，我们表明所有EBV感染的RS细胞并不局限于潜伏感染II型，有些属于潜伏感染I型。
