Shinagawa K, Ueno Y, Hu D, Ueda S, Sugii S
Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Japan.
J Vet Med Sci. 1996 Oct;58(10):1027-9. doi: 10.1292/jvms.58.10_1027.
The HEp-2 vacuolation factor (or cereulide) produced by Bacillus cereus isolated from vomiting-type food poisoning, which is supposed to induce emesis, was found to give mouse and suncus lethality after intravenous and intraperitoneal administration. The emetic activity of the factor was also found to be resistant to heating at 121 degrees C for 15 min, exposure to pH 2 and 11, and to digestion with proteolytic enzymes such as pepsin and trypsin. These findings suggest that the cereulide produced by B. cereus is stable in the digestive tracts, induce emesis, and show lethal activity leading to cellular damage.
从呕吐型食物中毒中分离出的蜡样芽孢杆菌所产生的HEp-2空泡形成因子（或蜡样芽胞杆菌溶血素）被认为可诱发呕吐，经静脉和腹腔注射后发现其对小鼠和麝鼩具有致死性。该因子的催吐活性还被发现对121℃加热15分钟、暴露于pH值2和11的环境以及用胃蛋白酶和胰蛋白酶等蛋白水解酶消化均具有抗性。这些发现表明，蜡样芽孢杆菌产生的蜡样芽胞杆菌溶血素在消化道中稳定，可诱发呕吐，并具有导致细胞损伤的致死活性。
