Cheng L L, Lean C L, Bogdanova A, Wright S C, Ackerman J L, Brady T J, Garrido L
Department of Radiology, Massachusetts General Hospital, Charlestown 02129, USA.
Magn Reson Med. 1996 Nov;36(5):653-8. doi: 10.1002/mrm.1910360502.
Proton NMR spectroscopy has proven useful in the detection of cancer in lymph node tissue. However, due to the high fat content of this type of tissue, 2D 1H COSY measurements (requiring acquisition times of 4-5 h or longer) are necessary to obtain the spectral information necessary for diagnosis. T2-filtered proton magic-angle spinning (MAS) NMR spectroscopy provides 1D spectra of lymph nodes in approximately 20 min with sufficient spectral resolution allowing for identification of changes in cellular chemistry due to the presence of malignant cells. MAS data from lymph nodes of five control and six rats with mammary adenocarcinoma (R13762) demonstrated increases in the signal intensity of resonances associated primarily with lactate (delta = 4.12 ppm) P < 0.0004, creatines/lysine (delta = 3.04 ppm) P < 0.0032, and glutamate/ glutamine (delta = 2.36 ppm) P < 0.0002 in metastatic compared with normal lymph nodes. The infiltration of lymph nodes by malignant cells is an important prognostic factor for many cancers. The rapid assessment of node tissue without the introduction of sampling errors (inherent in currently employed histological procedures) would allow postoperative therapy decisions to be made more efficiently.
质子核磁共振波谱已被证明在检测淋巴结组织中的癌症方面很有用。然而，由于这类组织中脂肪含量高，需要进行二维氢化学位移相关谱（2D 1H COSY）测量（采集时间需要4至5小时或更长）才能获得诊断所需的光谱信息。T2滤波质子魔角旋转（MAS）核磁共振波谱能在约20分钟内提供淋巴结的一维谱，其光谱分辨率足以识别由于恶性细胞的存在而导致的细胞化学变化。来自五只对照大鼠和六只患有乳腺腺癌（R13762）大鼠的淋巴结的MAS数据表明，与正常淋巴结相比，转移淋巴结中主要与乳酸（δ = 4.12 ppm）相关的共振信号强度增加（P < 0.0004），肌酸/赖氨酸（δ = 3.04 ppm）相关的共振信号强度增加（P < 0.0032），谷氨酸/谷氨酰胺（δ = 2.36 ppm）相关的共振信号强度增加（P < 0.0002）。恶性细胞对淋巴结的浸润是许多癌症的一个重要预后因素。在不引入采样误差（当前采用的组织学程序中固有的误差）的情况下快速评估淋巴结组织，将有助于更高效地做出术后治疗决策。
