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The isolation and analysis of polysomes and ribosomal RNA from cells growing in monolayer culture.

作者信息

Thomas G, Siegmann M, Bowman P D, Gordon J

出版信息

Exp Cell Res. 1977 Sep;108(2):253-8. doi: 10.1016/s0014-4827(77)80032-3.

DOI:10.1016/s0014-4827(77)80032-3
PMID:891639
Abstract
摘要

相似文献

1
The isolation and analysis of polysomes and ribosomal RNA from cells growing in monolayer culture.从单层培养生长的细胞中分离和分析多核糖体及核糖体RNA。
Exp Cell Res. 1977 Sep;108(2):253-8. doi: 10.1016/s0014-4827(77)80032-3.
2
Fractionation of membrane-bound polysomes, free polysomes, and nuclei from tissue-cultured cells.
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3
Isolation of messenger ribonucleoproteins from HeLa cells by affinity chromatography on poly (U) sepharose.通过在聚（U）琼脂糖上进行亲和层析从HeLa细胞中分离信使核糖核蛋白。
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4
A general method for the large-scale isolation of polysomes and messenger RNA applied to MOPC 21 mouse myeloma tumors.一种用于大规模分离多核糖体和信使核糖核酸的通用方法，应用于MOPC 21小鼠骨髓瘤肿瘤。
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5
The isolation of RNA from mammalian cells.从哺乳动物细胞中分离RNA。
Methods Cell Biol. 1973;7:1-22. doi: 10.1016/s0091-679x(08)61769-0.
6
The nature of histone f1 isolated from polysomes.从多核糖体中分离出的组蛋白F1的性质。
Biochemistry. 1973 Jan 16;12(2):237-45. doi: 10.1021/bi00726a010.
7
Recovery of microquantities of subcellular particles by ultracentrifugation in automatic pipet tips.通过在自动移液器吸头中进行超速离心回收微量亚细胞颗粒。
Anal Biochem. 1980 Dec;109(2):354-6. doi: 10.1016/0003-2697(80)90659-4.
8
A correlation between the polysome pattern and the synthesis of transfer RNA in Chang's liver cells.
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Isolation of active polyribosomes from the cytoplasm, mitochondria and chloroplasts of Euglena gracilis.从纤细裸藻的细胞质、线粒体和叶绿体中分离活性多核糖体。
Biochem J. 1972 Jun;128(2):353-65. doi: 10.1042/bj1280353.
10
Sequential Sepharose chromatographic isolation of polysomes and polysomal RNAs depleted in nuclear RNA from Xenopus.非洲爪蟾多核糖体及核RNA缺失的多核糖体RNA的连续琼脂糖凝胶色谱分离法
Nucleic Acids Res. 1979 Aug 10;6(11):3685-701. doi: 10.1093/nar/6.11.3685.

引用本文的文献

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Identification of a domain in human immunodeficiency virus type 1 rev that is required for functional activity and modulates association with subnuclear compartments containing splicing factor SC35.鉴定1型人类免疫缺陷病毒（HIV-1）Rev蛋白中一个功能活性所需的结构域，该结构域可调节与含有剪接因子SC35的核内亚区室的结合。
J Virol. 2000 Dec;74(24):11899-910. doi: 10.1128/jvi.74.24.11899-11910.2000.
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Nuclear diacylglycerol is increased during cell proliferation in vivo.在体内细胞增殖过程中，细胞核二酰甘油水平升高。
Biochem J. 1993 Mar 15;290 ( Pt 3)(Pt 3):633-6. doi: 10.1042/bj2900633.
3
Rapamycin selectively represses translation of the "polypyrimidine tract" mRNA family.
雷帕霉素选择性抑制“多嘧啶序列”mRNA家族的翻译。
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4441-5. doi: 10.1073/pnas.91.10.4441.
4
Transcriptional and translational control of cytoplasmic proteins after serum stimulation of quiescent Swiss 3T3 cells.血清刺激静止的瑞士3T3细胞后细胞质蛋白的转录和翻译控制
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5712-6. doi: 10.1073/pnas.78.9.5712.
5
Ordered phosphorylation of 40S ribosomal protein S6 after serum stimulation of quiescent 3T3 cells.血清刺激静止的3T3细胞后40S核糖体蛋白S6的有序磷酸化。
Proc Natl Acad Sci U S A. 1983 Feb;80(4):926-30. doi: 10.1073/pnas.80.4.926.
6
Polyribosomes in isolated liver cells. Preparative procedures, effects of incubation and correlation with protein synthesis.分离肝细胞中的多核糖体。制备方法、孵育效果及与蛋白质合成的相关性。
Biochem J. 1980 Jan 15;186(1):35-45. doi: 10.1042/bj1860035.
7
Translational control of mRNA expression during the early mitogenic response in Swiss mouse 3T3 cells: identification of specific proteins.瑞士小鼠3T3细胞早期促有丝分裂反应过程中mRNA表达的翻译调控：特定蛋白质的鉴定
J Cell Biol. 1986 Dec;103(6 Pt 1):2137-44. doi: 10.1083/jcb.103.6.2137.
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Regulation of protein kinase activities in PC12 pheochromocytoma cells.PC12嗜铬细胞瘤细胞中蛋白激酶活性的调节
EMBO J. 1986 Dec 20;5(13):3441-7. doi: 10.1002/j.1460-2075.1986.tb04667.x.
9
The polyphosphoinositide cycle exists in the nuclei of Swiss 3T3 cells under the control of a receptor (for IGF-I) in the plasma membrane, and stimulation of the cycle increases nuclear diacylglycerol and apparently induces translocation of protein kinase C to the nucleus.多磷酸肌醇循环存在于瑞士3T3细胞的细胞核中，受质膜上一种（胰岛素样生长因子-I）受体的调控，该循环的激活会增加细胞核中二酰甘油的含量，并明显诱导蛋白激酶C转位至细胞核。
EMBO J. 1991 Nov;10(11):3207-14. doi: 10.1002/j.1460-2075.1991.tb04883.x.
10
The Rev protein of human immunodeficiency virus type 1 promotes polysomal association and translation of gag/pol and vpu/env mRNAs.人类免疫缺陷病毒1型的Rev蛋白促进gag/pol和vpu/env信使核糖核酸的多核糖体结合及翻译。
Mol Cell Biol. 1992 Mar;12(3):1375-86. doi: 10.1128/mcb.12.3.1375-1386.1992.