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多磷酸肌醇循环存在于瑞士3T3细胞的细胞核中，受质膜上一种（胰岛素样生长因子-I）受体的调控，该循环的激活会增加细胞核中二酰甘油的含量，并明显诱导蛋白激酶C转位至细胞核。

The polyphosphoinositide cycle exists in the nuclei of Swiss 3T3 cells under the control of a receptor (for IGF-I) in the plasma membrane, and stimulation of the cycle increases nuclear diacylglycerol and apparently induces translocation of protein kinase C to the nucleus.

作者信息

Divecha N, Banfić H, Irvine R F

机构信息

Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.

出版信息

EMBO J. 1991 Nov;10(11):3207-14. doi: 10.1002/j.1460-2075.1991.tb04883.x.

DOI:10.1002/j.1460-2075.1991.tb04883.x
PMID:1655412
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC453044/
Abstract

When Swiss 3T3 cells are treated with Insulin-like Growth Factor I, a rapid decrease in the mass of polyphosphoinositol lipids (phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate) occurs within the nuclei, with a concomitant increase in nuclear diacylglycerol and translocation of protein kinase C to the nuclear region. This is in contrast to the effects of the regulatory peptide, bombesin, which causes similar inositol lipid changes in the plasma membrane, has no effect on nuclear inositide levels and causes a translocation of protein kinase C to post-nuclear membranes. These results suggest the existence of a discrete nuclear polyphosphoinositide signalling system entirely distinct from the well-known plasma membrane-located system, which is under regulatory control by cell surface-located receptors.

摘要

当用胰岛素样生长因子I处理瑞士3T3细胞时，细胞核内的多磷酸肌醇脂质（磷脂酰肌醇4-磷酸和磷脂酰肌醇4,5-二磷酸）质量迅速下降，同时核二酰基甘油增加，蛋白激酶C转位至核区域。这与调节肽蛙皮素的作用相反，蛙皮素可使质膜中的肌醇脂质发生类似变化，对核肌醇磷脂水平无影响，并导致蛋白激酶C转位至核后膜。这些结果表明存在一个完全不同于位于质膜的著名系统的离散核多磷酸肌醇信号系统，该系统受位于细胞表面的受体调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36fe/453044/92eb82d8ee57/emboj00109-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36fe/453044/58d3129b324c/emboj00109-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36fe/453044/92eb82d8ee57/emboj00109-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36fe/453044/58d3129b324c/emboj00109-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36fe/453044/92eb82d8ee57/emboj00109-0091-a.jpg

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1
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本文引用的文献

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A simple method for the isolation and purification of total lipides from animal tissues.一种从动物组织中分离和纯化总脂质的简单方法。
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Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin warts.定量超分辨率显微镜揭示了人类健康皮肤和皮肤疣中核磷脂酰肌醇4,5-二磷酸纳米级分布的差异。
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