Mano H, Shibuya N, Nakadaira H, Ohta T, Ishizu T, Yamazaki O, Takagi S, Endoh K, Yamamoto M, Hirohata T
Department of Hygiene and Preventive Medicine, Niigata University School of Medicine, Japan.
Mutat Res. 1996 Oct 1;370(3-4):203-8. doi: 10.1016/s0165-1218(96)00077-8.
In order to elucidate the genotoxicological characteristics of the Japanese diet, the mutagenicity of 24-h duplicate of the diet samples were investigated. The mutagenicity of blue rayon extract was examined in the Ames Salmonella/microsome assay. Thirty-two (91.4%) of 35 samples revealed mutagenicity toward Salmonella typhimurium TA98 in the presence of S9 mix. The mutagenic activities showed significant correlations with the consumption rates of broiled fish (r = 0.517, p = 0.0021) and broiled meat (r = 0.494, p = 0.0036). In other test conditions, 6 (17.1%), 5 (14.3%) and 8 (22.9%) samples were mutagenic to Salmonella typhimurium TA98 without S9 mix, TA100 with S9 mix and TA100 without S9 mix, respectively. Findings in the present study suggest that high consumption of broiled fish and broiled meat are important as the source of mutagens/carcinogens in the Japanese diet. In the present study, however, biological inference of these findings could not be made in relation to the occurrence of cancers, especially of the gastric cancer, which is the most prevalent form of cancer in Japan.
为了阐明日本饮食的遗传毒理学特征，对饮食样本的24小时复制品的致突变性进行了研究。在艾姆斯沙门氏菌/微粒体试验中检测了蓝色人造丝提取物的致突变性。35个样本中的32个（91.4%）在存在S9混合物的情况下对鼠伤寒沙门氏菌TA98显示出致突变性。致突变活性与烤鱼（r = 0.517，p = 0.0021）和烤肉（r = 0.494，p = 0.0036）的食用率呈显著相关。在其他测试条件下，分别有6个（17.1%）、5个（14.3%）和8个（22.9%）样本在无S9混合物时对鼠伤寒沙门氏菌TA98、有S9混合物时对TA100以及无S9混合物时对TA100具有致突变性。本研究结果表明，大量食用烤鱼和烤肉是日本饮食中诱变剂/致癌物的重要来源。然而，在本研究中，无法就这些发现与癌症尤其是胃癌（日本最常见的癌症形式）的发生进行生物学推断。
