Brandes G, Reale E, Messina A
Laboratory of Cell Biology and Electron Microscopy, Hannover Medical School, Germany.
Virchows Arch. 1996 Oct;429(2-3):165-72. doi: 10.1007/BF00192439.
The effect of externally applied mechanical stress was investigated by thin section electron microscopy of the microvessels in the unaffected palmar fascia in the carpal tunnel syndrome and in patients with Dupuytren's contracture before and after application of a continuous elongation device. In the unaffected palmar fascia the microfilaments of the endothelial cells were connected to a few adherens junctions and focal contacts; stress fibres were absent. In the cord of Dupuytren's disease the microfilaments were increased in quantity. The length ratios of the connections with the lateral and basal cell membrane were significantly higher than in the control group and increased to an even greater extent in the continuously extended fascia. Stress fibres appeared in the endothelial cells of postcapillary venules in the nonextended cord and in the endothelium of both arterioles and venules after extension elongation. the numerous intermediate filaments and the rare microtubules remained unchanged in the endothelial cells of all palmar fasciae analysed. In the endothelial cells of the microvessels the mechanical stress applied from outside mainly affected the contractile component of the cytoskeleton.
通过对腕管综合征患者未受影响的掌腱膜以及掌挛缩症患者在使用连续伸展装置前后的掌腱膜微血管进行薄切片电子显微镜检查，研究了外部施加机械应力的影响。在未受影响的掌腱膜中，内皮细胞的微丝连接到一些黏附连接和焦点黏附；不存在应力纤维。在掌挛缩症的条索中，微丝数量增加。与细胞膜外侧和基底连接的长度比显著高于对照组，并且在持续伸展的筋膜中增加得更多。在未伸展的条索中，毛细血管后微静脉的内皮细胞出现应力纤维，在伸展后，小动脉和微静脉的内皮细胞中均出现应力纤维。在所有分析的掌腱膜的内皮细胞中，大量的中间丝和稀少的微管保持不变。在微血管的内皮细胞中，外部施加的机械应力主要影响细胞骨架的收缩成分。
