使用染色体特异性载体PCR对人类多基因家族进行基因组分析。
Genomic analysis of human multigene families using chromosome-specific vectorette PCR.
作者信息
Moynihan T P, Markham A F, Robinson P A
机构信息
Molecular Medicine Unit, University of Leeds, St James's University Hospital, UK.
出版信息
Nucleic Acids Res. 1996 Oct 15;24(20):4094-5. doi: 10.1093/nar/24.20.4094.
Abstract
We report a technique for the rapid determination of genomic structure of individual members of human interspersed multigene families which circumvents the requirement for genomic clone isolation. In this approach, vectorette libraries were constructed from human/rodent somatic cell hybrid DNA harbouring single members of the gene family. Using these libraries as PCR templates with nested gene-specific primers in combination with a common vectorette primer resulted in the amplification of gene-specific products suitable for the subsequent determination of intron/exon structure. We have applied this technique to characterise members of two gene families.
摘要
我们报告了一种快速确定人类散布多基因家族单个成员基因组结构的技术，该技术无需进行基因组克隆分离。在这种方法中，从小鼠体细胞杂交DNA构建载体文库，这些DNA含有该基因家族的单个成员。使用这些文库作为PCR模板，结合巢式基因特异性引物和通用载体引物，可扩增出适合后续确定内含子/外显子结构的基因特异性产物。我们已应用该技术对两个基因家族的成员进行了表征。
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本文引用的文献
1
Isolation of homologous gene sequences by vectorette cloning.通过载体引物克隆分离同源基因序列。
Biotechniques. 1995 Oct;19(4):550-2.
2
Characterization of a human ubiquitin-conjugating enzyme gene UBE2L3.人类泛素结合酶基因UBE2L3的特征分析
Mamm Genome. 1996 Jul;7(7):520-5. doi: 10.1007/s003359900155.
3
A human ubiquitin conjugating enzyme, L-UBC, maps in the Alzheimer's disease locus on chromosome 14q24.3.一种人类泛素结合酶L-UBC定位于14号染色体q24.3的阿尔茨海默病基因座上。
Mamm Genome. 1995 Oct;6(10):725-31. doi: 10.1007/BF00354295.
4
Candidate gene for the chromosome 1 familial Alzheimer's disease locus.1号染色体家族性阿尔茨海默病位点的候选基因。
Science. 1995 Aug 18;269(5226):973-7. doi: 10.1126/science.7638622.
5
Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease.早发性家族性阿尔茨海默病中一个携带错义突变基因的克隆
Nature. 1995 Jun 29;375(6534):754-60. doi: 10.1038/375754a0.
6
Identification, molecular cloning, and characterization of the chromosome 12 breakpoint cluster region of uterine leiomyomas.子宫平滑肌瘤12号染色体断点簇区域的鉴定、分子克隆及特征分析。
Genes Chromosomes Cancer. 1994 Oct;11(2):106-18. doi: 10.1002/gcc.2870110207.
7
A novel exon in the cystic fibrosis transmembrane conductance regulator gene activated by the nonsense mutation E92X in airway epithelial cells of patients with cystic fibrosis.囊性纤维化跨膜传导调节因子基因中的一个新外显子，由囊性纤维化患者气道上皮细胞中的无义突变E92X激活。
J Clin Invest. 1994 Apr;93(4):1852-9. doi: 10.1172/JCI117172.
8
A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones.一种从酵母人工染色体（YAC）克隆中分离末端序列的新颖、快速方法。
Nucleic Acids Res. 1990 May 25;18(10):2887-90. doi: 10.1093/nar/18.10.2887.
9
Vectorette PCR: a novel approach to genomic walking.载体引物PCR：一种基因组步移的新方法。
PCR Methods Appl. 1991 Aug;1(1):39-42. doi: 10.1101/gr.1.1.39.
10
Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications.预防PCR前的错配引物和引物二聚体形成可改善低拷贝数扩增。
Nucleic Acids Res. 1992 Apr 11;20(7):1717-23. doi: 10.1093/nar/20.7.1717.
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