Cardoso A I, Blixenkrone-Moller M, Fayolle J, Liu M, Buckland R, Wild T F
INSERM Unit 404 Immunity and Vaccination, Institut Pasteur de Lyon, France.
Virology. 1996 Nov 15;225(2):293-9. doi: 10.1006/viro.1996.0603.
We have evaluated the DNA vaccination strategy for measles virus (MV) hemagglutinin (HA) and nucleoprotein (NP) genes. Plasmids encoding either the MV, HA, or NP proteins inoculated intramuscularly into Balb/c mice induced both humoral and CTL class I restricted responses. Antibody responses were not increased by multiple inoculations. The major antibody isotype induced by both the HA and NP was IgG2a consistent with a Th1 response. In contrast, immunization with a plasmid which directed the synthesis of a partially secreted form of HA gave mainly IgG1 antibodies. When the amount of DNA was reduced for the HA plasmid (1 or 10 microg/animal), although the antibody was not induced, a CTL response was observed.
我们评估了针对麻疹病毒（MV）血凝素（HA）和核蛋白（NP）基因的DNA疫苗接种策略。将编码MV、HA或NP蛋白的质粒肌肉注射到Balb/c小鼠体内，可诱导体液免疫和I类主要组织相容性复合体（MHC）限制性细胞毒性T淋巴细胞（CTL）反应。多次接种并未增强抗体反应。HA和NP诱导的主要抗体亚型均为IgG2a，这与Th1反应一致。相比之下，用一种指导合成部分分泌形式HA的质粒进行免疫主要产生IgG1抗体。当降低HA质粒的DNA用量（每只动物1或10微克）时，尽管未诱导出抗体，但观察到了CTL反应。
