粪肠球菌226中稳定隐蔽质粒的特性鉴定与序列分析及稳定克隆载体的构建
Characterization and sequence analysis of a stable cryptic plasmid from Enterococcus faecium 226 and development of a stable cloning vector.
作者信息
Wyckoff H A, Barnes M, Gillies K O, Sandine W E
机构信息
Department of Microbiology, Oregon State University, Corvallis 97331-3804, USA.
出版信息
Appl Environ Microbiol. 1996 Apr;62(4):1481-6. doi: 10.1128/aem.62.4.1481-1486.1996.
Abstract
A small cryptic plasmid, pMBB1, isolated from Enterococcus faecium 226 was characterized. The plasmid contained an extremely stable replicon which has limited homology to the lactococcal plasmid pCI305. Sequence analysis of the replicon detected one open reading frame of 822 bp capable of encoding a 32-kDa protein. No detectable single-stranded intermediates were found for the replicon, suggesting that pMBB1 may be included in the same family as pCI305, although pCI305 exhibits a more narrow host range. A small stably maintained vector able to replicate in a variety of lactic acid bacteria, containing a large multiple cloning region, was constructed by using the pMBB1 replicon.
摘要
对从粪肠球菌226中分离出的一个小的隐蔽质粒pMBB1进行了表征。该质粒含有一个极其稳定的复制子，与乳球菌质粒pCI305具有有限的同源性。对该复制子的序列分析检测到一个822 bp的开放阅读框，能够编码一种32 kDa的蛋白质。未发现该复制子有可检测到的单链中间体，这表明pMBB1可能与pCI305属于同一家族，尽管pCI305表现出更窄的宿主范围。通过使用pMBB1复制子构建了一个能够在多种乳酸菌中复制的、含有大的多克隆区域的小的稳定维持载体。
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