Markakis P K
Laboratory of Animal Nutrition, Aristotle University, Thessaloniki, Greece.
J AOAC Int. 1996 Mar-Apr;79(2):375-9.
This method was developed to separate, detect, and quantitate oxytetracycline (OTC) or chlortetracycline hydrochloride (CTC.HCl) in animal feeds in the presence of 11 other drugs: 3 nitrofurans, 2 macrolide antibiotics, 3 sulfonamides, 2 coccidiostatics, and 1 antibacterial growth promoter. OTC or CTC.HCl was separated from coexisting drugs and detected by thin-layer chromatography, then quantitated microbiologically by the agar diffusion method. Analysis of 125 experimental animal feed samples fortified at 5 levels (7.5-400 ppm) with OTC or CTC.HCl and at 1 level (50 ppm) with the rest of the drugs, respectively, gave a limit of quantitation of 1.25 or 0.625 ppm, a recovery of 90.6 or 92.9%, and a coefficient of variation of 2.9-6.1 or 2.3-4.4%.
该方法用于在存在其他11种药物（3种硝基呋喃类、2种大环内酯类抗生素、3种磺胺类、2种抗球虫药和1种抗菌生长促进剂）的情况下，分离、检测和定量动物饲料中的土霉素（OTC）或盐酸金霉素（CTC·HCl）。OTC或CTC·HCl与共存药物分离后，通过薄层色谱法进行检测，然后采用琼脂扩散法进行微生物定量。分别对125个用OTC或CTC·HCl以5个水平（7.5 - 400 ppm）以及其余药物以1个水平（50 ppm）进行强化的实验动物饲料样品进行分析，得到的定量限为1.25或0.625 ppm，回收率为90.6%或92.9%，变异系数为2.9 - 6.1或2.3 - 4.4%。
