采用薄层色谱法和微生物学法在存在其他药物的情况下测定动物饲料中的四环素。
Determination of tetracyclines in animal feeds in the presence of other drugs by thin-layer chromatography and microbiological method.
作者信息
Markakis P K
机构信息
Laboratory of Animal Nutrition, Aristotle University, Thessaloniki, Greece.
出版信息
J AOAC Int. 1996 Mar-Apr;79(2):375-9.
This method was developed to separate, detect, and quantitate oxytetracycline (OTC) or chlortetracycline hydrochloride (CTC.HCl) in animal feeds in the presence of 11 other drugs: 3 nitrofurans, 2 macrolide antibiotics, 3 sulfonamides, 2 coccidiostatics, and 1 antibacterial growth promoter. OTC or CTC.HCl was separated from coexisting drugs and detected by thin-layer chromatography, then quantitated microbiologically by the agar diffusion method. Analysis of 125 experimental animal feed samples fortified at 5 levels (7.5-400 ppm) with OTC or CTC.HCl and at 1 level (50 ppm) with the rest of the drugs, respectively, gave a limit of quantitation of 1.25 or 0.625 ppm, a recovery of 90.6 or 92.9%, and a coefficient of variation of 2.9-6.1 or 2.3-4.4%.
该方法用于在存在其他11种药物（3种硝基呋喃类、2种大环内酯类抗生素、3种磺胺类、2种抗球虫药和1种抗菌生长促进剂）的情况下，分离、检测和定量动物饲料中的土霉素（OTC）或盐酸金霉素（CTC·HCl）。OTC或CTC·HCl与共存药物分离后，通过薄层色谱法进行检测，然后采用琼脂扩散法进行微生物定量。分别对125个用OTC或CTC·HCl以5个水平（7.5 - 400 ppm）以及其余药物以1个水平（50 ppm）进行强化的实验动物饲料样品进行分析，得到的定量限为1.25或0.625 ppm，回收率为90.6%或92.9%，变异系数为2.9 - 6.1或2.3 - 4.4%。
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