Sedgley C M, Samaranayake L P, Darvell B W
Oral Biology Unit, Faculty of Dentistry, University of Hong Kong, Hong Kong.
APMIS. 1996 Jul-Aug;104(7-8):583-90. doi: 10.1111/j.1699-0463.1996.tb04915.x.
The adherence of 12 oral isolates and 4 type strains of Enterobacteriaceae (equally representing Enterobacter cloacae, Klebsiella pneumoniae, Escherichia coli and Citrobacter freundii) to HeLa cell monolayers following five different incubation conditions (sucrose, D-mannose, serum, MEM and Candida albicans GDH 1957) was investigated. Incubation with sucrose and D-mannose resulted in the greatest and least adherence, respectively. The presence of preadherent C. albicans GDH 1957 on the HeLa cells tended to enhance the adherence of certain strains of E. cloacae and C. freundii, but had no overall impact on Enterobacteriaceae adherence. While heterogeneity of behaviour existed between strains within species, E. cloacae was the most, and K. pneumoniae the least, adherent species irrespective of incubation conditions. Haemagglutination assays indicated the presence of mannose-resistant type 1 fimbriae associated with all Enterobacteriaceae. In clinical terms, the variations in adherence properties observed in vitro may contribute to an understanding of the different prevalence rates of oral Enterobacteriaceae reported in the literature.
研究了12株口腔分离株和4株肠杆菌科标准菌株（分别代表阴沟肠杆菌、肺炎克雷伯菌、大肠埃希菌和弗氏柠檬酸杆菌）在5种不同培养条件（蔗糖、D-甘露糖、血清、MEM和白色念珠菌GDH 1957）下对HeLa细胞单层的黏附情况。与蔗糖和D-甘露糖孵育分别导致最大和最小的黏附。HeLa细胞上预先黏附的白色念珠菌GDH 1957倾向于增强某些阴沟肠杆菌和弗氏柠檬酸杆菌菌株的黏附，但对肠杆菌科的整体黏附没有影响。虽然种内菌株之间存在行为异质性，但无论培养条件如何，阴沟肠杆菌是黏附性最强的菌种，肺炎克雷伯菌是黏附性最弱的菌种。血凝试验表明所有肠杆菌科均存在与甘露糖抗性1型菌毛相关的情况。从临床角度来看，体外观察到的黏附特性差异可能有助于理解文献中报道的口腔肠杆菌科不同的流行率。
