针对猴疱疹病毒（B病毒）的病毒特异性和交叉反应性单克隆抗体的特性分析

Characterization of virus-specific and cross-reactive monoclonal antibodies to Herpesvirus simiae (B virus).

作者信息

Blewett E L, Black D, Eberle R

机构信息

Department of Veterinary Parasitology, Microbiology and Public Health, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078-2006, USA.

出版信息

J Gen Virol. 1996 Nov;77 ( Pt 11):2787-93. doi: 10.1099/0022-1317-77-11-2787.

DOI:10.1099/0022-1317-77-11-2787

PMID:8922473

Abstract

A panel of 13 monoclonal antibodies (MAbs) was produced that detect B virus (BV) proteins. Several of these MAbs were highly specific for BV, while the remainder cross-reacted in varying degrees with the other primate alphaherpesviruses. Utilizing western blot and radioimmunoprecipitation analysis, the MAbs were found to detect at least four distinct BV-infected cell antigens, several of which were composed of multiple polypeptides. One target antigen has been identified as the BV glycoprotein B (gB) homologue and was recognized by both virus-specific and cross-reactive MAbs. Although gB is an essential protein, none of the anti-gB MAbs neutralized infectious virus.

摘要

制备了一组13种单克隆抗体（MAb），用于检测B病毒（BV）蛋白。其中几种单克隆抗体对BV具有高度特异性，而其余的则与其他灵长类α疱疹病毒有不同程度的交叉反应。利用蛋白质印迹法和放射免疫沉淀分析，发现这些单克隆抗体可检测到至少四种不同的BV感染细胞抗原，其中几种由多种多肽组成。一种靶抗原已被鉴定为BV糖蛋白B（gB）同源物，可被病毒特异性和交叉反应性单克隆抗体识别。尽管gB是一种必需蛋白，但没有一种抗gB单克隆抗体能中和感染性病毒。