欧洲对一种用于鉴定耐甲氧西林金黄色葡萄球菌的商业系统的多中心评估。
European multicentre evaluation of a commercial system for identification of methicillin-resistant Staphylococcus aureus.
作者信息
Zambardi G, Fleurette J, Schito G C, Auckenthaler R, Bergogne-Berezin E, Hone R, King A, Lenz W, Lohner C, Makristhatis A, Marco F, Müller-Serieys C, Nonhoff C, Phillips I, Rohner P, Rotter M, Schaal K P, Struelens M, Viebahn A
机构信息
Laboratoire de Microbiologie, Hôpital Edouard Herriot, Lyon, France.
出版信息
Eur J Clin Microbiol Infect Dis. 1996 Sep;15(9):747-9. doi: 10.1007/BF01691964.
A commercial system for the rapid detection of methicillin-resistant Staphylococcus aureus, the BBL Crystal MRSA test (C-MRSA ID; Becton Dickinson, USA), was evaluated prospectively and compared with a polymerase chain reaction test for the presence of the mecA gene. Ten European centres tested a total of 676 isolates of Staphylococcus aureus from blood cultures. The system correctly identified 661 (97.8%) isolates within 4 h. All but three mecA gene-negative isolates (99.4% specificity) yielded a negative C-MRSA ID reaction, and 158 of 170 mecA gene-positive isolates were accurately detected (92.9% sensitivity). After repeated testing of discrepant results, sensitivity and specificity increased to 99% and 100%, respectively.
一种用于快速检测耐甲氧西林金黄色葡萄球菌的商业系统——BBL Crystal MRSA检测法（C-MRSA ID；美国BD公司），进行了前瞻性评估，并与检测mecA基因存在情况的聚合酶链反应检测法进行了比较。十个欧洲中心共检测了来自血培养的676株金黄色葡萄球菌分离株。该系统在4小时内正确鉴定出661株（97.8%）分离株。除了三株mecA基因阴性分离株外（特异性为99.4%），所有分离株的C-MRSA ID反应均为阴性，170株mecA基因阳性分离株中的158株被准确检测到（敏感性为92.9%）。在对差异结果进行重复检测后，敏感性和特异性分别提高到99%和100%。
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