评估BBL Crystal MRSA ID系统用于检测金黄色葡萄球菌对苯唑西林的耐药性。
Evaluation of the BBL Crystal MRSA ID System for detection of oxacillin resistance in Staphylococcus aureus.
作者信息
Kampf G, Lecke C, Cimbal A K, Weist K, Rüden H
机构信息
Institut für Hygiene, Umweltmedizin und Arbeitsmedizin, Freie Universität Berlin, Germany.
出版信息
J Clin Pathol. 1999 Mar;52(3):225-7. doi: 10.1136/jcp.52.3.225.
AIMS
To evaluate the BBL Crystal MRSA ID System for detection of oxacillin resistance in Staphylococcus aureus.
METHODS
52 methicillin resistant S aureus (MRSA) from five different countries and 85 methicillin susceptible S aureus (MSSA) were included. The species was confirmed by tube coagulation and detection of the clumping factor using the Staphaurex Plus. Clonal non-identity of the MRSA isolates was shown by pulsed field gel electrophoresis. MIC values (oxacillin) were determined using the Etest. Polymerase chain reaction was carried out to detect the mecA gene. The BBL Crystal MRSA ID System was carried out according to the manufacturer's instructions.
RESULTS
The BBL Crystal MRSA ID System showed fluorescence in 45 of 52 MRSA (sensitivity 86.5%; negative predicitive value 92.2%), and the specificity was 97.6% (positive predicitive value 95.7%). Two of seven MRSA that failed to show fluorescence had MIC values (oxacillin) of 4 mg/litre.
CONCLUSIONS
The BBL Crystal MRSA ID System is a valuable test for detecting oxacillin resistance in S aureus. Its major advantage is the short time (4-5 hours) required to perform the test when organisms are grown on tryptic soy agar or sheep blood agar. Difficulties may arise in borderline resistant isolates.
目的
评估BBL Crystal MRSA ID系统检测金黄色葡萄球菌对苯唑西林耐药性的能力。
方法
纳入来自五个不同国家的52株耐甲氧西林金黄色葡萄球菌（MRSA）和85株甲氧西林敏感金黄色葡萄球菌（MSSA）。通过试管凝集试验以及使用Staphaurex Plus检测凝聚因子来确认菌种。通过脉冲场凝胶电泳显示MRSA分离株的克隆非同一性。使用Etest测定（苯唑西林的）MIC值。进行聚合酶链反应以检测mecA基因。按照制造商的说明使用BBL Crystal MRSA ID系统。
结果
BBL Crystal MRSA ID系统在52株MRSA中的45株中显示出荧光（敏感性86.5%；阴性预测值92.2%），特异性为97.6%（阳性预测值95.7%）。七株未显示荧光的MRSA中有两株的（苯唑西林）MIC值为4毫克/升。
结论
BBL Crystal MRSA ID系统是检测金黄色葡萄球菌对苯唑西林耐药性的一项有价值的检测方法。其主要优点是当细菌在胰蛋白胨大豆琼脂或羊血琼脂上生长时，进行检测所需的时间较短（4 - 5小时）。对于临界耐药菌株可能会出现困难。
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