长期乙醇摄入对大鼠胰腺对2-脱氧-D-葡萄糖引起的中枢迷走神经刺激反应的影响。

Effects of chronic ethanol consumption on pancreatic response to central vagal stimulation by 2-deoxy-D-glucose in the rat.

作者信息

Chariot J, Rozé C, de La Tour J, Souchard M, Hollande E, Vaille C, Debray C

出版信息

Digestion. 1977;15(5):425-37. doi: 10.1159/000198030.

DOI:10.1159/000198030

Abstract

Male Wistar rats, fed a standard normal laboratory diet, drank ad libitum a 20% ethanol solution for 3 months. Basal and 2-deoxy-D-glucose (2DG) stimulated pancreatic secretion were measured. Basal secretion of sodium (-30%, p less than 0.001), bicarbonate (-35%, p less than 0.001) and total protein (-35%, p less than 0.001) were depressed in alcoholic versus control rats. Pancreatic response was identical in both groups with the smaller dose of 2DG, dose related in controls, and identical for both doses in alcohol-fed rats. The response was thus significantly smaller in alcohol-fed rats than in controls for the larger dose of 2DG (p less than 0.01). Pancreatic concentration and contents of amylase, trypsinogen, chymotrypsinogen and lipase were all decreased in alcoholic versus control rats (40-60%, p less than 0.001). These results are consistent with the hypothesis of functional modifications in pancreatic cholinergic innervation in alcohol-fed rats.

摘要

雄性Wistar大鼠喂养标准正常实验室饮食，随意饮用20%乙醇溶液3个月。测量基础状态和2-脱氧-D-葡萄糖（2DG）刺激后的胰腺分泌。与对照大鼠相比，酒精喂养大鼠的基础钠分泌（-30%，p<0.001）、碳酸氢盐分泌（-35%，p<0.001）和总蛋白分泌（-35%，p<0.001）均降低。两组对较小剂量2DG的胰腺反应相同，对照组呈剂量相关性，酒精喂养大鼠对两种剂量的反应相同。因此，对于较大剂量的2DG，酒精喂养大鼠的反应明显小于对照组（p<0.01）。与对照大鼠相比，酒精喂养大鼠胰腺中淀粉酶、胰蛋白酶原、糜蛋白酶原和脂肪酶的浓度及含量均降低（40-60%，p<0.001）。这些结果与酒精喂养大鼠胰腺胆碱能神经支配功能改变的假说一致。