Aspenberg P, Jeppsson C, Wang J S, Boström M
Department of Orthopedics, Lund University Hospital, Sweden.
Bone. 1996 Nov;19(5):499-503. doi: 10.1016/s8756-3282(96)90257-4.
We compared the ability of transforming growth factor beta 1 (TGF beta 1) and bone morphogenetic protein 2 (BMP-2) to increase the penetration distances for fibrous tissue and bone into porous coralline hydroxyapatite. Forty-four rats received pairs of titanium bone chambers implanted bilaterally in their proximal tibiae. These chambers allow tissue ingrowth from one end of a long cylinder, so that ingrowth distances along the cylinder axis can be measured. Since bone never grows as far as fibrous or undifferentiated mesenchymal tissue, we measured both total tissue ingrowth and bone ingrowth distances. All chambers were implanted with cylinders of porous hydroxyapatite (Interpore 200). The hydroxyapatite cylinders were treated with either 1, 33, or 1000 ng of TGF beta 1 or BMP-2 prior to implantation, and compared with untreated contralateral control implants at 6 weeks. The bone ingrowth distance into the porous hydroxyapatite showed a trend toward inhibition with TGF beta 1 as compared to untreated controls (p < 0.08), and there was a negative correlation between TGF beta 1 dose and ingrowth distances for both bone (p < 0.01) and total tissue (p < 0.01). BMP-2 greatly increased bone (p < 0.001) and total tissue (p < 0.001) ingrowth distances.
我们比较了转化生长因子β1（TGFβ1）和骨形态发生蛋白2（BMP - 2）增加纤维组织和骨向多孔珊瑚羟基磷灰石内生长距离的能力。44只大鼠双侧胫骨近端植入成对的钛质骨腔。这些骨腔允许组织从长圆柱体的一端向内生长，从而可以测量沿圆柱体轴线的生长距离。由于骨的生长距离从不及纤维性或未分化的间充质组织，我们测量了总的组织向内生长距离和骨向内生长距离。所有骨腔均植入多孔羟基磷灰石圆柱体（Interpore 200）。在植入前，将羟基磷灰石圆柱体用1、33或1000 ng的TGFβ1或BMP - 2进行处理，并在6周时与未处理的对侧对照植入物进行比较。与未处理的对照相比，TGFβ1使骨向多孔羟基磷灰石内的生长距离呈抑制趋势（p < 0.08），并且TGFβ1剂量与骨（p < 0.01）和总的组织（p < 0.01）的生长距离之间均呈负相关。BMP - 2显著增加了骨（p < 0.001）和总的组织（p < 0.001）的生长距离。
