Hernández A, Belda F J, Domínguez J, Matas L, Gimenez M, Caraballo M, Ramil C, Ausina V
Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Universidad Autónoma de Barcelona, Spain.
J Hosp Infect. 1996 Nov;34(3):223-8. doi: 10.1016/s0195-6701(96)90070-5.
The antiviral activities of sodium dichloroisocyanurate (NaDCC) and a commercial product (Solprogel 2%) against human immunodeficiency virus type 1 (HIV-1) were investigated using a quantitative suspension test method. Solprogel is a compound that contains NaDCC and a biodegradable polymer of acrylic acid. Viral suspensions were prepared containing 3.2 x 10(6) tissue culture infective dose 50 (TCID50) in culture media. Syncytium formation in the MT-2 line and HIV antigen p24 on the supernatant of the cultures were used to determine viral titre. Results indicate that satisfactory disinfection (1000-fold reduction in 5 min) can be achieved using NaDCC and Solprogel at concentrations of 100 and 120 ppm available chlorine, respectively.
采用定量悬浮试验方法，研究了二氯异氰尿酸钠（NaDCC）和一种商品（Solprogel 2%）对1型人类免疫缺陷病毒（HIV-1）的抗病毒活性。Solprogel是一种含有NaDCC和丙烯酸可生物降解聚合物的化合物。在培养基中制备含有3.2×10⁶组织培养感染剂量50（TCID50）的病毒悬液。利用MT-2细胞系中的合胞体形成以及培养物上清液中的HIV抗原p24来测定病毒滴度。结果表明，分别使用有效氯浓度为100 ppm和120 ppm的NaDCC和Solprogel可实现令人满意的消毒效果（5分钟内病毒滴度降低1000倍）。
