Abakumova O Iu, Kutsenko N G, Fedorova L M, Podobed O V, Gavril'chak A V, Shekhter A B, Kovaleva G G, Popov A A, Li V S, Karagiulian S R, Mitina V Kh, Kliashchitskiĭ B A
Vestn Ross Akad Med Nauk. 1996(5):36-41.
The effects of hepatotropic growth factors (HGFs) and phospholipid drugs on the recovery of functions and the regeneration of the rat liver were studied in CC14-induced toxic damage and after partial hepatectomy (PHE). HGFs isolated from the cytoplasmic cells of the regenerating liver, as well as from the liver of the animals given prodigiozan and from the media taken after culturing the explants of the regenerating liver were found to stimulate DNA synthesis and hepatocytic proliferation following PHE and in the cirrhotic liver. Prodigiozan was shown to induce the formation of HGFs not only in the rat liver following PHE, but in the liver of intact animals. It was established that the covalently binding complex of albumin and bilirubin stimulated the synthesis of proteins and DNA in the regenerating liver, but non-covalently binding complex inhibited these processes. When CC14 was administered to the animals, the two complexes enhanced the reparative synthesis of DNA, without changing the level of replicating synthesis, the non-covalently binding complex completely eliminating the single-strand breaks in DNA. Phospholipid agents containing soybean and sunflower phosphatidylcholines increased the synthesis of RNA and albumin, which were decreased due to exposure to CC14 and had the property of stimulating the synthesis of total DNA and considerably enhancing that of mitochondrial DNA.
研究了促肝细胞生长因子（HGFs）和磷脂类药物对四氯化碳诱导的大鼠肝脏毒性损伤及部分肝切除（PHE）后肝脏功能恢复和再生的影响。从再生肝脏的细胞质细胞、给予灵菌红素的动物肝脏以及培养再生肝脏外植体后的培养基中分离出的HGFs，被发现可刺激PHE后及肝硬化肝脏中的DNA合成和肝细胞增殖。结果表明，灵菌红素不仅在PHE后的大鼠肝脏中诱导HGFs的形成，在未受处理的动物肝脏中也可诱导其形成。已证实，白蛋白与胆红素的共价结合复合物可刺激再生肝脏中的蛋白质和DNA合成，但非共价结合复合物则抑制这些过程。当给动物注射四氯化碳时，这两种复合物可增强DNA的修复合成，而不改变复制合成水平，非共价结合复合物能完全消除DNA中的单链断裂。含有大豆和向日葵磷脂酰胆碱的磷脂制剂可增加RNA和白蛋白的合成，这两种物质因接触四氯化碳而减少，并且该磷脂制剂具有刺激总DNA合成并显著增强线粒体DNA合成的特性。
