利用光寻址电位传感器测定抗DNA抗体的解离常数和浓度
Determination of dissociation constant and concentration of an anti-DNA antibody by using the light-addressable potentiometric sensor.
作者信息
Dill K, Fraser C, Blomdahl J A, Olson J D
机构信息
Molecular Devices Corporation, Sunnyvale, CA 94089, USA.
出版信息
J Biochem Biophys Methods. 1996 Jan 11;31(1-2):17-21. doi: 10.1016/0165-022x(95)00023-k.
PMID:8926334
Abstract
The most sensitive assay possible is desired for quantitation of total DNA and DNA probe binding. To reduce background signal, which may reduce assay sensitivity, it is desirable to employ low antibody concentrations. This requires the use of a specific antibody with a high affinity for DNA.
摘要
对于总DNA和DNA探针结合的定量分析，需要尽可能灵敏的检测方法。为了降低可能会降低检测灵敏度的背景信号，宜采用低抗体浓度。这就需要使用对DNA具有高亲和力的特异性抗体。
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