Thacore H R, Cunningham R K, Nakeeb S, Zaleski M B
Department of Microbiology, State University of New York at Buffalo, 14214-3078, USA.
J Clin Lab Immunol. 1995;46(3):97-109.
Healthy, adult C57BL/6Kh mice of both sexes were transfused with blood or blood products from syngeneic donors with retrovirus (LP-BM5)-induced lymphoproliferative disease. The disease produced in the recipients 8 weeks after transfusion was characterized by splenomegaly, disseminated lymphadenopathy, leukopenia with neutrophilia, abrogation of the primary immune response to SRBC, decreased in vitro proliferation of spleen cells co-stimulated with phorbol ester and IL-2 or ionomycin and abrogation of synergistic effect of the co stimulators. Quantitative analysis of the blood or blood products used for transfusion show that a single transfusion of 0.2 ml of PBS containing 0.2 mu 1 of whole blood or 2 microliters of plasma or 400 Ficoll-isolated peripheral blood mononuclear cells was sufficient for the inducing the disease. The results suggest that the retroviruses were present in preparations of peripheral blood mononuclear cells and plasma of mice with the disease. However, the latter was 10-fold less efficient in inducing the disease. Transfusion of 1.8 x 10(6) isolated erythrocytes failed to induce the disease suggesting a marginal role, if any, in transmission of the disease via transfusion of these cells. Thus, a simple, reliable and reproducible method for propagation of the murine lymphoproliferative disease in the laboratory has been elaborated. These results also point to some important differences with regard to blood transfusion between human and murine AIDS.
将来自同基因供体且患有逆转录病毒（LP - BM5）诱导的淋巴细胞增生性疾病的血液或血液制品，输注给健康的成年C57BL/6Kh雌雄小鼠。输注8周后，受者产生的疾病特征为脾肿大、弥漫性淋巴结病、白细胞减少伴中性粒细胞增多、对绵羊红细胞的初次免疫反应消失、佛波酯和白细胞介素 - 2或离子霉素共同刺激的脾细胞体外增殖减少以及共刺激剂协同效应消失。对用于输注的血液或血液制品进行定量分析表明，单次输注0.2毫升含0.2微升全血的磷酸盐缓冲液、2微升血浆或400个经菲可分离的外周血单核细胞足以诱发该疾病。结果表明，逆转录病毒存在于患有该疾病小鼠的外周血单核细胞和血浆制剂中。然而，后者诱发疾病的效率低10倍。输注1.8×10⁶个分离的红细胞未能诱发该疾病，这表明这些细胞在通过输血传播疾病方面即使有作用也是微不足道的。因此，已经制定了一种在实验室中传播小鼠淋巴细胞增生性疾病的简单、可靠且可重复的方法。这些结果也指出了人类艾滋病和小鼠艾滋病在输血方面的一些重要差异。
